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AMB Express

Table 2 Effect of different variables on cellulase production through CCD

From: Carboxymethyl cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology

Run#

Substrate conc. (X1)

Inoculum size (X2)

Yeast extract  % (X3)

pH (X4)

Enzyme activity (IU/ml)

Residual value

Observed

Predicted

1

0.5

3

0.8

5.5

0.32

0.39

−0.074

2

1.5

3

0.8

6.5

1.60

1.61

−0.012

3

1.0

4

1.0

5.0

1.10

1.19

−0.09

4

1.5

3

0.2

5.5

2.01

2.09

−0.08

5

1.5

3

0.8

5.5

1.57

1.88

−0.31

6

2.5

3

0.8

5.5

2.57

2.83

−0.26

7

1.0

2

1.0

6.0

1.40

1.45

−0.05

8

1.5

3

1.2

5.5

1.96

2.11

−0.15

9

1.5

1

0.8

5.5

2.10

2.30

−0.20

10

1.0

2

1.0

5.0

1.60

1.65

−0.05

11

1.0

2

0.6

5.0

1.30

1.05

0.25

12

2.0

4

0.6

6.0

2.70

2.41

0.28

13

1.0

4

0.6

6.0

1.20

1.45

−0.25

14

2.0

4

1.0

6.0

1.84

1.97

−0.13

15

2.0

2

1.0

6.0

2.50

2.36

0.13

16

1.5

3

0.8

4.5

1.70

2.02

−0.32

17

2.0

4

0.6

5.0

2.80

2.63

0.16

18

1.0

4

1.0

6.0

1.20

0.93

0.26

19

1.0

2

0.6

6.0

1.60

1.46

0.13

20

1.0

4

1.0

5.0

1.40

1.19

0.20

21

2.0

4

1.0

5.0

2.89

2.80

0.09

22

1.5

5

0.8

5.5

1.83

1.96

−0.13

23

2.0

2

0.6

5.0

2.41

2.44

−0.03

24

1.5

3

0.8

5.5

2.10

1.88

0.21

25

1.0

4

0.6

5.0

1.20

1.10

0.09

26

2.0

2

1.0

5.0

3.50

3.13

0.36

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