Skip to main content

Table 2 Effect of different variables on cellulase production through CCD

From: Carboxymethyl cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology

Run# Substrate conc. (X1) Inoculum size (X2) Yeast extract  % (X3) pH (X4) Enzyme activity (IU/ml) Residual value
Observed Predicted
1 0.5 3 0.8 5.5 0.32 0.39 −0.074
2 1.5 3 0.8 6.5 1.60 1.61 −0.012
3 1.0 4 1.0 5.0 1.10 1.19 −0.09
4 1.5 3 0.2 5.5 2.01 2.09 −0.08
5 1.5 3 0.8 5.5 1.57 1.88 −0.31
6 2.5 3 0.8 5.5 2.57 2.83 −0.26
7 1.0 2 1.0 6.0 1.40 1.45 −0.05
8 1.5 3 1.2 5.5 1.96 2.11 −0.15
9 1.5 1 0.8 5.5 2.10 2.30 −0.20
10 1.0 2 1.0 5.0 1.60 1.65 −0.05
11 1.0 2 0.6 5.0 1.30 1.05 0.25
12 2.0 4 0.6 6.0 2.70 2.41 0.28
13 1.0 4 0.6 6.0 1.20 1.45 −0.25
14 2.0 4 1.0 6.0 1.84 1.97 −0.13
15 2.0 2 1.0 6.0 2.50 2.36 0.13
16 1.5 3 0.8 4.5 1.70 2.02 −0.32
17 2.0 4 0.6 5.0 2.80 2.63 0.16
18 1.0 4 1.0 6.0 1.20 0.93 0.26
19 1.0 2 0.6 6.0 1.60 1.46 0.13
20 1.0 4 1.0 5.0 1.40 1.19 0.20
21 2.0 4 1.0 5.0 2.89 2.80 0.09
22 1.5 5 0.8 5.5 1.83 1.96 −0.13
23 2.0 2 0.6 5.0 2.41 2.44 −0.03
24 1.5 3 0.8 5.5 2.10 1.88 0.21
25 1.0 4 0.6 5.0 1.20 1.10 0.09
26 2.0 2 1.0 5.0 3.50 3.13 0.36