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Table 1 S. cerevisiae strains and plasmids used in this study

From: Molecular breeding of Saccharomyces cerevisiae with high RNA content by harnessing essential ribosomal RNA transcription regulator

Name Description
Strain
 SH6471 MATα ura3851 his3200 leu2trp163 ade2-661
 SH8836 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP [pRRN5]
 SH8894 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP13
 SH8895 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP14
 SH8896 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP15
 SH8897 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP16
 SH8898 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP17
 SH8899 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP18
 SH8900 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP19
 SH8904 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP20
 SH8905 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1-loxP SUP24
 SH30025 Ura+ transformants of SH8894 with pRRN5
 SH30026 Ura+ transformants of SH8895 with pRRN5
 SH30027 Ura+ transformants of SH8896 with pRRN5
 SH30028 Ura+ transformants of SH8897 with pRRN5
 SH30029 Ura+ transformants of SH8898 with pRRN5
 SH30030 Ura+ transformants of SH8899 with pRRN5
 SH30031 Ura+ transformants of SH8900 with pRRN5
 SH30035 Ura+ transformants of SH8904 with pRRN5
 SH30036 Ura+ transformants of SH8905 with pRRN5
 TK1 MATα ura3851 his3200 leu2trp163 ade2-661rrn5::loxP-CgTRP1fob1::HIS3 [pRRN5]
 TK2 ∆fob1::HIS3 disruptant of SH8894
 TK3 ∆fob1::HIS3 disruptant of SH8895
 TK4 ∆fob1::HIS3 disruptant of SH8896
 TK5 ∆fob1::HIS3 disruptant of SH8897
 TK6 ∆fob1::HIS3 disruptant of SH8898
 TK7 ∆fob1::HIS3 disruptant of SH8899
 TK8 ∆fob1::HIS3 disruptant of SH8900
 TK9 ∆fob1::HIS3 disruptant of SH8904
 TK10 ∆fob1::HIS3 disruptant of SH8905
 TK11 Ura+ transformants of TK2 with pRRN5
 TK12 Ura+ transformants of TK5 with pRRN5
Plasmid
 pRRN5 YCp-URA3-RRN5 (A centromere type plasmid harboring functional RRN5 gene marked with URA3 gene)
 p2453 pUC18 plasmid harboring FOB1 gene disruption cassette (a kind gift from T. Kobayashi)