Table 1 S. cerevisiae strains and plasmids used in this study

Strain

 SH6471

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661

 SH8836

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP [pRRN5]

 SH8894

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP13

 SH8895

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP14

 SH8896

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP15

 SH8897

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP16

 SH8898

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP17

 SH8899

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP18

 SH8900

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP19

 SH8904

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP20

 SH8905

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1-loxP SUP24

 SH30025

Ura⁺ transformants of SH8894 with pRRN5

 SH30026

Ura⁺ transformants of SH8895 with pRRN5

 SH30027

Ura⁺ transformants of SH8896 with pRRN5

 SH30028

Ura⁺ transformants of SH8897 with pRRN5

 SH30029

Ura⁺ transformants of SH8898 with pRRN5

 SH30030

Ura⁺ transformants of SH8899 with pRRN5

 SH30031

Ura⁺ transformants of SH8900 with pRRN5

 SH30035

Ura⁺ transformants of SH8904 with pRRN5

 SH30036

Ura⁺ transformants of SH8905 with pRRN5

 TK1

MATα ura3∆851 his3∆200 leu2∆ trp1∆63 ade2-661 ∆rrn5::loxP-CgTRP1 ∆fob1::HIS3 [pRRN5]

 TK2

∆fob1::HIS3 disruptant of SH8894

 TK3

∆fob1::HIS3 disruptant of SH8895

 TK4

∆fob1::HIS3 disruptant of SH8896

 TK5

∆fob1::HIS3 disruptant of SH8897

 TK6

∆fob1::HIS3 disruptant of SH8898

 TK7

∆fob1::HIS3 disruptant of SH8899

 TK8

∆fob1::HIS3 disruptant of SH8900

 TK9

∆fob1::HIS3 disruptant of SH8904

 TK10

∆fob1::HIS3 disruptant of SH8905

 TK11

Ura⁺ transformants of TK2 with pRRN5

 TK12

Ura⁺ transformants of TK5 with pRRN5

Plasmid

 pRRN5

YCp-URA3-RRN5 (A centromere type plasmid harboring functional RRN5 gene marked with URA3 gene)

 p2453

pUC18 plasmid harboring FOB1 gene disruption cassette (a kind gift from T. Kobayashi)