Fig. 5From: Molecular breeding of Saccharomyces cerevisiae with high RNA content by harnessing essential ribosomal RNA transcription regulatorTotal RNA content of ∆fob1Sup mutants with pRRN5. Intracellular total RNA content of ∆fob1Sup13 and ∆fob1Sup16 mutants with pRRN5. Cells were cultivated in YPDA liquid medium and harvested at middle exponential phase. Intracellular RNA content was measured by PCA method as described in “Materials and Methods” section. The values are the means and standard deviations of results from three independent experiments. Significant difference of ∆fob1Sup16 from SH8836 was confirmed by Student’s t test (P < 0.01)Back to article page