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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Molecular breeding of Saccharomyces cerevisiae with high RNA content by harnessing essential ribosomal RNA transcription regulator

Fig. 3

Determination of rDNA copy number of Sup mutants. a Sup mutants were subjected to Southern blotting using 5S rRNA gene as a probe after pulsed field gel electrophoresis. The curly bracket indicates expanded rDNA repeats in Sup mutants. Broad bands indicate that cell cultures are heterogeneous state. The arrow indicates rDNA repeat in SH8836 control strain. b Sup mutants harboring pRRN5 were subjected to Southern blotting using 5S rRNA gene as a probe after pulsed field gel electrophoresis. c Precise determination of rDNA copy number in Sup mutants by quantitative real-time PCR. A primer pair RT-PCR_18S-F and RT-PCR_18S-R were used for detection of rDNA unit. ACT1 gene was used as the standard for calculation of rDNA copy number. Grey bars Sup mutants, white bars Sup mutants harboring pRRN5. The sequence of oligonucleotide primers used in this experiment is listed in Additional file 1: Table S1

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