Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China

AMB Express

Table 3 C/N ratios and their effects on biomass, lipid production, yield and productivity of newly isolated oleaginous yeast strains

Treatment	Glucose (g/l)	Peptone (g/l)	Yeast extract (g/l)	Biomass (g/l)	Lipid (g/l)	Yield (g/g)	Productivity (g/l–h)
Rhodosporidium TJUWZ4
20C^a	12.05	0.5	1.5	8.22 ± 0.64	1.60 ± 0.41	0.19	0.027
20N^b	48	2	6	11.83 ± 0.22	2.86 ± 0.22	0.24	0.048
80	48	0.5	1.5	12.4 ± 0.78	5.40 ± 1.42	0.44	0.090
120C^a	72.3	0.50	1.5	11.54 ± 0.54	3.52 ± 0.56	0.31	0.059
120N^b	48	0.33	0.99	13.32 ± 1.19	3.86 ± 0.05	0.29	0.064
Cryptococcus TJUWZA11
20C^a	14	2	0	8.92 ± 0.73	0.91 ± 0.25	0.10	0.015
20N^b	56	8	0	8.2 ± 0.43	0.75 ± 0.02	0.09	0.013
80	56	2	0	9.64 ± 0.41	1.19 ± 0.11	0.12	0.020
120C^a	84	2	0	9.38 ± 0.81	1.02 ± 0.18	0.11	0.017
120N^b	56	1.33	0	10.16 ± 0.78	1.54 ± 0.43	0.15	0.026

C/N calculation formula: \({{{\text{C}}/{\text{N}} = \left[ {\text{Glu}} \right] \times 0. 4 { }\left( {{\text{g}} - {\text{C}}/{\text{g}} - {\text{Glu}}} \right)} \mathord{\left/ {\vphantom {{{\text{C}}/{\text{N}} = \left[ {\text{Glu}} \right] \times 0. 4 { }\left( {{\text{g}} - {\text{C}}/{\text{g}} - {\text{Glu}}} \right)} {\left\{ {\left( {\left[ {\text{Pep}} \right]{\text{ x }}0. 1 4 { }\left( {{\text{g}} - {\text{N}}/{\text{g}} - {\text{Pep}}} \right)} \right) + \left( {\left[ {\text{YE}} \right]{\text{ x }}0. 1 1 4 { }\left( {{\text{g wt}}.{\text{ N in YE}}} \right)} \right)} \right\}}}} \right. \kern-0pt} {\left\{ {\left( {\left[ {\text{Pep}} \right]{\text{ x }}0. 1 4 { }\left( {{\text{g}} - {\text{N}}/{\text{g}} - {\text{Pep}}} \right)} \right) + \left( {\left[ {\text{YE}} \right]{\text{ x }}0. 1 1 4 { }\left( {{\text{g wt}}.{\text{ N in YE}}} \right)} \right)} \right\}}}\)
^aC means that altering C/N ratio by changing carbon source contents at a constant concentration of nitrogen source
^bN means that altering C/N ratio by changing nitrogen source contents at a constant concentration of carbon source