Skip to main content
AMB Express

Table 1 Spore harvests in lids of plastic Petri-dishes after incubation of mushrooms in upside-down or upside-top position relative to the lids

From: Bulk isolation of basidiospores from wild mushrooms by electrostatic attraction with low risk of microbial contaminations

Species

No of mushrooms per situation

Part used

No of samples

Incubation time (h)

Spore harvests

Mushroom upside-down

Mushroom upside-top

Experimental set-up 1: mushroom caps laid onto wet paper

 Coprinellus domesticus

3

1

3

18

4.8 ± 0.3 × 106

6.7 ± 0.3 × 107

 Panaeolus cinctulus

2

1

2

18

4.5 ± 0.9 × 106

4.0 ± 0.8 × 107

 Schizophyllum commune

3

1

3

18

7.3 ± 1.0 × 106

8.3 ± 0.7 × 107

 Tubaria hiemalis

5

1

5

18

3.5 ± 1.2 × 104

8.1 ± 0.9 × 104

Experimental set-up 2: mushroom caps stuck into water agar

 Coprinellus domesticus

1

1

1

18

2.4 × 106

2.1 × 107

 Coprinellus micaceus a

2

1/2

3

8

1.1 ± 0.1 × 105

0.9 ± 0.2 × 105

    

18

1.2 ± 0.1 × 105

4.0 ± 0.2 × 105

 Coprinopsis atramentaria

2

1/2

3

18

2.1 ± 0.7 × 106

2.2 ± 1.0 × 107

 Lacrymaria spec.

2

1/2

3

18

2.7 ± 1.2 × 105

2.8 ± 1.1 × 106

 Paneolus papilionaceus

1

1

1

18

4.1 × 105

3.5 × 106

 Pluteus spec.

1

1/2

1

18

6.7 × 104

2.7 × 105

 Schizophyllum commune b

3

1

3

18

4.7 ± 1.8 × 106

6.1 ± 2.1 × 107

5.6 ± 0.8 × 104

2.6 ± 0.2 × 105

  1. aIn one series of experiments, the incubation time was stopped at 8 h to avoid faults through onset of cap autolysis. In all other instances where inkcaps were used, there was no obvious cap autolysis
  2. bSpore data in the upper line are from freshly collected mushrooms, data in the lower line are from revived dry fruiting bodies after 15 days open storage incubation at RT
Back to article page