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Fig. 6 | AMB Express

Fig. 6

From: Bulk isolation of basidiospores from wild mushrooms by electrostatic attraction with low risk of microbial contaminations

Fig. 6

Bacterial contaminations in spore solutions of Paneolus cinctulus (black columns), Coprinellus micaceus (white columns), and Coprinellus domesticus mushrooms (grey columns). After upside-down incubation of mushrooms for basidiospore harvests from plastic lids (Fig. 2a, b), each 50 µl of spore solutions were plated onto MEA medium, incubated at 25 °C for 3 days and bacterial colonies were counted. Two different basidiospore solutions were always tested per time point for a species. For P. cinctulus and C. micaceus, these came always from the two halves of a same mushroom which were incubated for spore collection in parallel in different Petri-dishes. For C. domesticus, the each two parallel spore collections came from different mushrooms. Bacterial colonies in the P. cinctulus sample B, 20 h (shown in the figure at furthest distance to the observer) were uncountable why 106 bacteria was used in the figure as an arbitrary estimate

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