Table 1 Primer sequences used in this study
Target | Locus (access number) | Primer type | Name | Primer sequence 5′→3′ (added restriction site is underlined) | Melting temperature (°C) | Expected size (bp) |
|---|---|---|---|---|---|---|
pDONR-221 | ccdB (U51588.1) | Encoding gene/colony PCR primer | ccdB-ecoRI | CGg/aattcAAGCCAGATAACAGTATGCG | 60 | 675 |
ccdB-pstI | AActgca/gACTGGCTGTGTAT | |||||
pELMO | ccdB (U51588.2) | Sequencing primer | ccdBsec-F | TGCAGTTTAAGGTTTACACC | 56 | 161 bp+ (DNA insert) |
ccdBsec-R | CACCACCGGGTAAAGTTC | |||||
Plasmodium falciparum | CSP (XM_001351086) | Encoding gene/colony PCR primer | F-NCOI | c/catggAGTGCTATGGAAGTTCGT | 62 | 246 |
R-XHO | CCGc/tcgagTCATGCATTTGGATCAGGATTAC | |||||
Plasmodium falciparum | MSP-1(XM_001352134) | Encoding gene/colony PCR primer | F-CT | CATGc/catggTAGTTGTATTACCCATTTTT | 55 | 512 |
R-CT | CCGc/tcgagTCAGATAACTTTTTTAATTGATTC | |||||
Plasmodium falciparum | EBA-175 (XM_001349171) | Encoding gene/colony PCR primer | F-NCO | CATGc/catggTATCCACTAAAGATGTATGTG | 54 | 891 |
EBARII-Stop | CCGc/tcgagTCATCCATCCGTACGAGTTTC | |||||
Neospora caninum | Nc5 (AY459289.1) | Encoding gene/colony PCR primer | Np21+ | CCCAGTGCGTCCAATCCTGTAGAC | 60 | 350 |
Np6+ | CTCGCCAGTCAACCTACGTCTTCT | |||||
Plasmodium vivax | ARNP (Pv_Sal1_chr10)-(828,231–828,827) | Encoding gene/colony PCR primer | PvARNP-D | ATGAAAAAAGTGGCCTCGTT | 54 | 597 |
PvARNP-R | AAGGTTGAAGAAAAATTTAAAAA | |||||
Plasmodium vivax | PvRON4 (KF378614) | Encoding gene primer | pvron4dir | CACAGTGCAACCATGTCTCG | 68 | ~2300 |
pvron4rev | GCAAGCTAATTTCACAAGTCTTC | |||||
Plasmodium vivax | PvRON4 (KF378614) | Colony PCR primer | pvron4intdir | CACAGTGCAACCATGTCTCG | 60 | 844 |
pvron4intrev | GCAAGCTAATTTCACAAGTCTTC | |||||
pGEM-T easy vector | Sequencing primer | SP6 | ATTTAGGTGACACTATAG | 54 | 177 bp+ (DNA insert) | |
T7 | AATACGACTCACTATAG | |||||