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Table 3 Detection of orf virus in clinical samples by conventional PCR and real-time PCR

From: Development of a SYBR Green I real-time PCR for the detection of the orf virus

Regions of Anhui province

Number of clinical samples

Conventional PCR positive (%)

Real-time PCR positive (%)

Feidong

15

60.00 (9/15)

86.67 (13/15)

Bozhou

16

62.50 (10/16)

81.25 (13/16)

Jinzhai

16

68.75 (11/16)

87.50 (14/16)

Shouxian

18

72.22 (13/18)

88.89 (16/18)

Total positivity

65

66.15 (43/65)

86.15 (56/65)