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Table 2 Intra- and inter-reproducibility assay

From: Development of a SYBR Green I real-time PCR for the detection of the orf virus

Category DNA standard (copies/µL) Mean (Ct) SD CV (%)
Intra-assay 1 × 107 15.09 0.017 0.11
1 × 105  21.65 0.025 0.12
1 × 103  28.55 0.030 0.11
1 × 101  35.17 0.075 0.21
Inter-assay 1 × 107  15.15 0.041 0.27
1 × 105  21.76 0.035 0.16
1 × 103  28.44 0.048 0.17
1 × 101  35.15 0.136 0.39