From: Development of a SYBR Green I real-time PCR for the detection of the orf virus
Category | DNA standard (copies/µL) | Mean (Ct) | SD | CV (%) |
---|---|---|---|---|
Intra-assay | 1 × 107 | 15.09 | 0.017 | 0.11 |
1 × 105 | 21.65 | 0.025 | 0.12 | |
1 × 103 | 28.55 | 0.030 | 0.11 | |
1 × 101 | 35.17 | 0.075 | 0.21 | |
Inter-assay | 1 × 107 | 15.15 | 0.041 | 0.27 |
1 × 105 | 21.76 | 0.035 | 0.16 | |
1 × 103 | 28.44 | 0.048 | 0.17 | |
1 × 101 | 35.15 | 0.136 | 0.39 |