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Table 2 Intra- and inter-reproducibility assay

From: Development of a SYBR Green I real-time PCR for the detection of the orf virus

Category

DNA standard (copies/µL)

Mean (Ct)

SD

CV (%)

Intra-assay

1 × 107

15.09

0.017

0.11

1 × 105 

21.65

0.025

0.12

1 × 103 

28.55

0.030

0.11

1 × 101 

35.17

0.075

0.21

Inter-assay

1 × 107 

15.15

0.041

0.27

1 × 105 

21.76

0.035

0.16

1 × 103 

28.44

0.048

0.17

1 × 101 

35.15

0.136

0.39