Fig. 1
From: Development of a SYBR Green I real-time PCR for the detection of the orf virus
Results of SYBR Green I real-time PCR for standard ORFV DNA. a Amplification curve. Ten-fold dilutions of standard DNA ranged from 1 × 108 to 1 × 101 copies/µL. b Standard curve. Dilutions ranged from 2 × 108 to 2 × 101 copies per reaction. Standard curve: y = − 3.345 × log (x) + 39.09; correlation coefficient: R2 = 0.999; reaction efficiency: Eff = 99.0%. c Melting curve. Dilutions ranged from 2 × 108 to 2 × 101 copies per reaction. Tm = 83.79 °C. d Specificity of SYBR Green I real-time PCR. Sample 1 orf virus, 2 goatpox virus, 3 sheeppox virus, 4 swine pseudorabies virus, 5 no template (negative control)