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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Fig. 3

FAR/FADO gene copy number determination in the selected TDR transformant. a Hybridization probe used for the Southern blot analysis; FADO fatty aldehyde deformylating oxygenase; TrpC_T terminator region of TrpC; Tef1_P promoter region of Tef1; FAR fatty acyl-ACP/CoA reductase; BamHI, NdeI, PstI restriction enzyme cut sites. b Southern blot analysis. Lane 1 contains 1 kb DNA ladder (size in base pairs); lanes 2–4 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the TDR transformant; lanes 5–7 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the parent strain

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