Fig. 3

a Pretreatment of the biomass with lysozyme (2 ml) and 5M H₂SO₄ (20 ml) at different temperatures. In the first two attempts biomass was first treated with acid and followed by hydrolysis with lysozyme at room temperature and 150 °C respectively. In the last three attempts biomass was first treated with lysozyme and then followed by acid hydrolysis at 25, 150 and 100 °C respectively. Data represents mean values of different experiments performed in triplicate ± standard deviation. b Reduced sugars contents (mM/ml) in the samples resulted from the pretreatment of algal biomass with lysozyme, acid hydrolysis and saccharification with invertase. Data are expressed as mean of values from experiments in triplicates ± standard deviation