Table 4 Constituent components, obtained from each thermophile genome, that can be coupled with α-subunit of the terminal oxygenase component in order to reconstitute functional RO system
From: Thermophilic bacteria are potential sources of novel Rieske non-heme iron oxygenases
Organism (NCBI accession no. of the putative RO terminal oxygenase α-subunit) | Protein annotation [accession no.] | Putative function |
|---|---|---|
Class A | ||
 Thermomicrobium roseum DSM 5159 (ACM06903) | 3-Phenylpropionate dioxygenase subunit beta [ACM06517] | Beta subunit |
Rieske protein [ACM07109] 4Fe–4S ferredoxin, iron–sulfur binding domain protein [ACM06911] | Ferredoxin | |
Ferredoxin–NADP reductase; thioredoxin reductase [ACM06977] Putidaredoxin reductase [ACM07176] Thioredoxin reductase [ACM06488] | Reductase | |
 Meiothermus ruber DSM 1279 (ADD29434) | Aromatic-ring-hydroxylating dioxygenase beta subunit [ADD29433] | Beta subunit |
Rieske (2Fe–2S) domain protein [ADD27546] | Ferredoxin | |
Dihydrolipoamide dehydrogenase [ADD29074] | Reductase | |
 Meiothermus timidus DSM 17022 (WP_026234685) | Aromatic-ring-hydroxylating dioxygenase subunit beta [WP_018466297] | Beta subunit |
Diguanylate cyclase [WP_026234838] | Ferredoxin | |
Dihydrolipoamide dehydrogenase [WP_026234742] Dihydrolipoamide dehydrogenase [WP_026234716] hypothetical protein [WP_018465799] | Reductase | |
 Thermus igniterrae ATCC 700962 (WP_018111968) | Aromatic-ring-hydroxylating dioxygenase subunit beta [WP_018111967] | Beta subunit |
 Thermus oshimai JL-2 (AFV77211) | Small subunit of phenylpropionate dioxygenase [AFV77212] | Beta subunit |
Ferredoxin subunit of nitrite reductase and ring-hydroxylating dioxygenase [AFV75193] | Ferredoxin | |
NAD(FAD)-dependent dehydrogenase [AFV75199] Dihydrolipoamide dehydrogenase [AFV77091] Flavoprotein oxygenase [WP_016329217] | Reductase | |
 Thermus scotoductus SA-01 (ADW21768) | 3-Phenylpropionate dioxygenase, subunit beta [ADW21767] | Beta subunit |
Ferredoxin subunit of phenylpropionate dioxygenase [ADW21026] 4Fe–4S Ferredoxin/formate dehydrogenase, nitrate-inducible, iron–sulfur subunit [ADW21629] | Ferredoxin | |
NADH oxidase/coenzyme A disulfide reductase [ADW21032] Phenol hydroxylase component B [ADW21668] | Reductase | |
 Thermus sp. CCB_US3_UF1 (AEV16357) | Naphthalene dioxygenase small subunit [AEV16358] | Beta subunit |
Initial dioxygenase ferredoxin subunit [AEV16981] | Ferredoxin | |
4-Hydroxybenzoate 3-monooxygenase [AEV16360] FAD-dependent pyridine nucleotide-disulfide oxidoreductase [AEV16975] 4-Hydroxyphenylacetate 3-monooxygenase reductase (Flav_red) [AEV16285] | Reductase | |
 Thermus thermophilus ATCC 33923 (WP_024119937) | NADH oxidase [WP_024119527] | Reductase |
 Alicyclobacillus hesperidum URH17-3-68 (WP_040289757) | Thioesterase superfamily protein [EJY55215] | Beta subunit |
Dihydropteridine reductase [WP_006448064] Dihydrolipoamide dehydrogenase [WP_006446492] NADH dehydrogenase [WP_006448257] Thioredoxin reductase [WP_006447474]; | Reductase | |
 Brevibacillus thermoruber PM1 (WP_035295329) | Aromatic-ring-hydroxylating dioxygenase [WP_035295713] | Beta subunit |
Dihydrolipoamide dehydrogenase [WP_035297436] | Reductase | |
 Geobacillus sp. JF8 (AGT33881) | Small subunit of biphenyl dioxygenase [AGT33882] | Beta subunit |
Nitrite reductase NAD(P)H small subunit [AGT32189] | Ferredoxin | |
Thioredoxin reductase [AGT33412] | Reductase | |
 Geobacillus thermoglucosidasius NBRC 107763 (GAJ45328) | Putative naphthalene 1,2-dioxygenase small subunit [GAJ45329] | Beta subunit |
Hypothetical protein GT2_34_00240 [GAJ45383] | Ferredoxin | |
Thioredoxin reductase [GAJ43870] Coenzyme A disulfide reductase [GAJ42754] Putative oxidoreductase [GAJ45381] | Reductase | |
Class B | ||
 Thermus thermophilus JL-18 (AFH40260) | Small subunit of phenylpropionate dioxygenase [AFH40261] | Beta subunit |
Ferredoxin subunit of nitrite reductase and ring-hydroxylating dioxygenase [AFH39800] | Ferredoxin | |
NAD(FAD)-dependent dehydrogenase [AFH39794] NAD(FAD)-dependent dehydrogenase [AFH40137] | Reductase | |
Class D | ||
 Anaerolinea thermophila UNI-1 (BAJ63376) | Putative ferredoxin [BAJ63818] | Ferredoxin |
Phytoene dehydrogenase [BAJ63379] | Reductase | |
 Meiothermus rufus DSM 22234 (WP_027881162) | Dehydrogenase [WP_027881168] | Reductase |
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (ACV59062) | Rieske (2Fe–2S) iron–sulphur domain protein [ACV58029] | Ferredoxin |
Arsenate reductase (thioredoxin) [ACV59045] Flavin reductase domain protein FMN-binding [ACV59470] Flavin reductase domain protein FMN-binding [ACV58921] | Reductase | |
 Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 (AEJ44076) | Rieske (2Fe–2S) domain protein [AEJ42957] | Ferredoxin |
Dihydrolipoamide dehydrogenase [AEJ42545] Flavin reductase domain protein FMN-binding protein [AEJ43919] | Reductase | |
 Alicyclobacillus acidoterrestris ATCC 49025 (EPZ45189) | Hypothetical protein N007_08015 [EPZ45711] Hypothetical protein [EPZ45711] | Reductase |
 Alicyclobacillus pomorum DSM 14955 (WP_035467417) | Dihydropteridine reductase [WP_035467306] | Reductase |
 Bacillus thermotolerans SGZ-8 (KKB35183) | Absent |  |
 Cohnella thermotolerans DSM 17683 (WP_027092788) | Ferredoxin [WP_027093665] | Ferredoxin |
 Coprothermobacter platensis DSM 11748 (WP_018963776) | 2-Enoate reductase [WP_018963737] Hypothetical protein [WP_018963777] | Reductase |
 Sulfobacillus thermosulfidooxidans ST (WP_051350961) | (2Fe–2S)-binding protein [WP_037913512] | Ferredoxin |
Hypothetical protein, partial [WP_040767264] | Reductase | |
 Thermoactinomycetaceae bacterium GD1 (WP_044639983) | Absent |  |
 Thermoanaerobacterium xylanolyticum LX-11 (AEF16296) | Rubredoxin-type Fe(Cys)4 protein [AEF16485] | Ferredoxin |
Pyruvate ferredoxin/flavodoxin oxidoreductase [AEF16297] CoA-disulfide reductase [AEF16270] Thioredoxin reductase [AEF16306] | Reductase | |
 Fervidobacterium pennivorans DSM 9078 (AFG35170) | Rubredoxin [AFG34604] | Ferredoxin |
2-Polyprenylphenol hydroxylase-like oxidoreductase [AFG34955] Phytoene dehydrogenase-like oxidoreductase [AFG35173] Thioredoxin-disulfide reductase [AFG35278] FAD binding protein [AFG35124] Thioredoxin reductase [AFG34984] Dihydrolipoamide dehydrogenase [AFG34941] Thioredoxin reductase [AFG35081] | Reductase | |
 Thermosipho africanus TCF52B (ACJ75179) | Rubredoxin [ACJ76024] | Ferredoxin |
Oxidoreductase/NADH: ubiquinone oxidoreductase, na translocating, f-subunit [ACJ75165] Oxidoreductase/tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA [ACJ75137] Ferredoxin/anaerobic glycerol 3-phosphate dehydrogenase [ACJ75136] | Reductase | |
 Thermotoga maritima MSB8 (AAD36358) | Rubredoxin [AGL49584] | Ferredoxin |
Oxidoreductase [AAD35836] | Reductase | |
 Thermotoga maritima MSB8 (AGL50271) | Rubredoxin [AGL49584] | Ferredoxin |
Oxidoreductase [AAD35836] | Reductase | |
 Thermotoga naphthophila RKU-10 (ADA67544) | Rubredoxin-type Fe(Cys)4 protein [ADA66551] | Ferredoxin |
FAD-dependent pyridine nucleotide-disulphide oxidoreductase [ADA67462] | Reductase | |
 Thermotoga sp. Mc24 (KHC91410) | Rubredoxin-type Fe(Cys)4 protein [KHC90328] | Ferredoxin |
Oxidoreductase [KHC90231] | Reductase | |
 Thermotoga sp. RQ2 (ACB09872) | Rubredoxin-type Fe(Cys)4 protein [ACB08629] | Ferredoxin |
FAD-dependent pyridine nucleotide-disulphide oxidoreductase [ACB08534] | Reductase | |
 Thermotoga sp. Xyl54 (KHC95729) | Rubredoxin-type Fe(Cys)4 protein [KHC96420] | Ferredoxin |
Class D* | ||
 Caldilinea aerophila DSM 14535 (BAL99910) | Hypothetical protein [BAL99023] | Ferredoxin |
Putative mercuric reductase [BAM01871] Putative flavin reductase [BAL99068] | Reductase | |
 Roseiflexus castenholzii DSM 13941 (ABU59830) | Rieske (2Fe–2S) domain protein [ABU56444] | Ferredoxin |
Dihydrolipoamide dehydrogenase [ABU58189] Flavin reductase domain protein FMN-binding [ABU60217] Flavin reductase domain protein FMN-binding [ABU58489] | Reductase | |
 Roseiflexus sp. RS-1 (WP_011955741) | Rieske (2Fe–2S) domain protein [ABQ89132] | Ferredoxin |
FAD-dependent pyridine nucleotide-disulphide oxidoreductase [ABQ91503] Dihydrolipoamide dehydrogenase [ABQ91367] Flavin reductase domain protein, FMN-binding [ABQ92660] Flavin reductase domain protein, FMN-binding [ABQ90950] | Reductase | |
 Sphaerobacter thermophilus DSM 20745 (ACZ37596) | Rieske (2Fe–2S) iron–sulphur domain protein [ACZ40633] | Ferredoxin |
FAD-dependent pyridine nucleotide-disulphide oxidoreductase [ACZ40632] Flavin reductase domain protein FMN-binding protein [ACZ39977] | Reductase | |
 Thermomicrobium roseum DSM 5159 (ACM04599) | Rieske protein [ACM07109] | Ferredoxin |
Xylene monooxygenase electron transfer subunit [ACM06651] Ferredoxin–NADP reductase; thioredoxin reductase [ACM06977] Putidaredoxin reductase [ACM07176] Thioredoxin reductase [ACM06488] | Reductase | |
 Meiothermus cerbereus DSM 11376 (WP_027876468) | Diguanylatecyclase [WP_027876040] | Ferredoxin |
Dihydrolipoyl dehydrogenase [WP_027876580] MFS transporter [WP_027877386] | Reductase | |
 Meiothermus chliarophilus DSM 9957 (WP_027893118) | Dihydrolipoyl dehydrogenase [WP_027893501] MFS transporter [WP_036218580] | Reductase |
 Meiothermus ruber DSM 1279 (AGK03951) | Dihydrolipoamide dehydrogenase [ADD29074] Flavin reductase domain-containing FMN-binding protein [AGK04562] | Reductase |
 Meiothermus rufus DSM 22234 (WP_027882714) | Dehydrogenase [WP_027881168] | Reductase |
 Meiothermus silvanus DSM 9946 (ADH64647) | Rieske (2Fe–2S) iron–sulfur domain protein [ADH62911] | Ferredoxin |
FAD-dependent pyridine nucleotide-disulfide oxidoreductase [ADH64702] Flavin reductase domain protein FMN-binding protein [ADH64172] | Reductase | |
 Meiothermus timidus DSM 17022 (WP_018466224) | Dihydrolipoamide dehydrogenase [WP_026234742] Dihydrolipoamide dehydrogenase [WP_026234716] Hypothetical protein [WP_018465799] | Reductase |