Table 3 The closest characterized homologue of each thermophilic RO terminal oxygenase α-subunit sequence obtained from RHObase
From: Thermophilic bacteria are potential sources of novel Rieske non-heme iron oxygenases
Organism (NCBI accession no. of the putative RO terminal oxygenase α-subunit) | Closest match obtained from RHObase | Accession no. | Identity (%) | Query coverage (%) |
|---|---|---|---|---|
Class A | ||||
 Thermomicrobium roseum DSM 5159 (ACM06903) | Biphenyl 2,3-dioxygenase (BphA1) from Geobacillus sp. JF8 | BAC79226 | 46.10 | 91 |
 Meiothermus ruber DSM 1279 (ADD29434) | Dibenzofuran dioxygenase (NarAa) from Rhodococcus opacus SAO101 | BAD02377 | 50.11 | 92 |
 Meiothermus timidus DSM 17022 (WP_026234685) | Indene dioxygenase (NidA) from Rhodococcus sp. I24 | AAD25395 | 49.89 | 98 |
 Thermus igniterrae ATCC 700962 (WP_018111968) | Indene dioxygenase (NidA) from Rhodococcus sp. I24 | AAD25395 | 51.48 | 99 |
 Thermus oshimai JL-2 (AFV77211) | Indene dioxygenase (NidA) from Rhodococcus sp. I24 | AAD25395 | 50.34 | 98 |
 Thermus scotoductus SA-01 (ADW21768) | Dibenzofuran dioxygenase (NarAa) from Rhodococcus opacus SAO101 | BAD02377 | 51.14 | 99 |
 Thermus sp. CCB_US3_UF1 (AEV16357) | Dibenzofuran dioxygenase (NarAa) from Rhodococcus opacus SAO101 | BAD02377 | 51.26 | 97 |
 Thermus thermophilus ATCC 33923 (WP_024119937) | Indene dioxygenase (NidA) from Rhodococcus sp. I24 | AAD25395 | 51.03 | 97 |
 Alicyclobacillus hesperidum URH17-3-68 (WP_040289757) | Dibenzofuran dioxygenase (NarAa) from Rhodococcus opacus SAO101 | BAD02377 | 43.02 | 93 |
 Brevibacillus thermoruber PM1 (WP_035295329) | Indene dioxygenase (NidA) from Rhodococcus sp. I24 | AAD25395 | 44.59 | 92 |
 Geobacillus sp. JF8 (AGT33881) | Biphenyl 2,3-dioxygenase (BphA1) from Geobacillus sp. JF8 | BAC79226 | 100.00 | 100 |
 Geobacillus thermoglucosidasius NBRC 107763 (GAJ45328) | Indene dioxygenase (NidA) from Rhodococcus sp. I24 | AAD25395 | 45.81 | 93 |
Class B | ||||
 Thermus thermophilus JL-18 (AFH40260) | p-Cumate dioxygenase (CmtAb) from Pseudomonas putida F1 | AAB62285 | 45.37 | 94 |
Class D | ||||
 Anaerolinea thermophila UNI-1 (BAJ63376) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 32.04 | 31 |
 Meiothermus rufus DSM 22234 (WP_027881162) | Vanillate O-demethylase (VanA) from Pseudomonas sp. ATCC19151 | AAA26019 | 32.10 | 47 |
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (ACV59062) | Toluene-4-sulfonate methyl monooxygenase (TsaM2) from Comamonas testosteroni T-2 | AAK37996 | 32.45 | 32 |
 Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 (AEJ44076) | 3-Ketosteroid 9α-hydroxylase (KshA) from Rhodococcus opacus B-4 | BAH52700 | 30.54 | 54 |
 Alicyclobacillus acidoterrestris ATCC 49025 (EPZ45189) | Toluene-4-sulfonate methyl monooxygenase (TsaM2) from Comamonas testosteroni T-2 | AAK37996 | 30.22 | 48 |
 Alicyclobacillus pomorum DSM 14955 (WP_035467417) | 3-Chlorobenzoate-3,4/4,5-dioxygenase (CbaA) from Comamonas testosteroni BR60 | AAC45716 | 27.56 | 50 |
 Bacillus thermotolerans SGZ-8 (KKB35183) | Phthalate 4,5-dioxygenase (Pht3) from Pseudomonas putida | BAA02511 | 37.86 | 94 |
 Cohnella thermotolerans DSM 17683 (WP_027092788) | Phthalate 4,5-dioxygenase (OphA2) from Burkholderia cepacia DBO1 | AAD03558 | 36.24 | 97 |
 Coprothermobacter platensis DSM 11748 (WP_018963776) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 32.93 | 51 |
 Sulfobacillus thermosulfidooxidans ST (WP_051350961) | Toluene-4-sulfonate methyl monooxygenase (TsaM2) from Comamonas testosteroni T-2 | AAK37996 | 30.12 | 46 |
 Thermoactinomycetaceae bacterium GD1 (WP_044639983) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 34.78 | 54 |
 Thermoanaerobacterium xylanolyticum LX-11 (AEF16296) | Vanillate O-demethylase (VanA) from Pseudomonas sp. HR199 | CAA72287 | 31.84 | 48 |
 Fervidobacterium pennivorans DSM 9078 (AFG35170) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 34.13 | 47 |
 Thermosipho africanus TCF52B (ACJ75179) | Vanillate O-demethylase (VanA) from Pseudomonas sp. HR199 | CAA72287 | 33.92 | 46 |
 Thermotoga maritima MSB8 (AAD36358) | 3-Chlorobenzoate-3,4/4,5-dioxygenase (CbaA) from Comamonas testosteroni BR60 | AAC45716 | 25.35 | 65 |
 Thermotoga maritima MSB8 (AGL50271) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 32.72 | 64 |
 Thermotoga naphthophila RKU-10 (ADA67544) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 32.32 | 73 |
 Thermotoga sp. Mc24 (KHC91410) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 32.72 | 73 |
 Thermotoga sp. RQ2 (ACB09872) | 3-Chlorobenzoate-3,4/4,5-dioxygenase (CbaA) from Comamonas testosteroni BR60 | AAC45716 | 25.35 | 65 |
 Thermotoga sp. Xyl54 (KHC95729) | Dicamba O-demethylase (DdmC) from Stenotrophomonas maltophilia DI-6 | AAV53699 | 32.72 | 73 |
Class D* | ||||
 Caldilinea aerophila DSM 14535 (BAL99910) | Phenylpropionate dioxygenase from Phenylobacterium zucineum HLK1a | WP_012521516 | 36.50 | 71 |
 Roseiflexus castenholzii DSM 13941(ABU59830) | (2Fe–2S)-binding protein from Parvibaculum lavamentivorans DS-1Ta | WP_011995073 | 33.92 | 75 |
 Roseiflexus sp. RS-1 (WP_011955741) | (2Fe–2S)-binding protein from Parvibaculum lavamentivorans DS-1Ta | WP_011995074 | 33.63 | 81 |
 Sphaerobacter thermophilus DSM 20745 (ACZ37596) | Aromatic oxygenase (GbcA) from Pseudomonas aeruginosa PAO1 | NP_254097 | 31.36 | 88 |
 Thermomicrobium roseum DSM 5159 (ACM04599) | Aromatic oxygenase (GbcA) from Pseudomonas aeruginosa PAO1 | NP_254097 | 29.47 | 91 |
 Meiothermus cerbereus DSM 11376 (WP_027876468) | (2Fe–2S)-binding protein from Parvibaculum lavamentivorans DS-1Ta | WP_011995073 | 26.91 | 80 |
 Meiothermus chliarophilus DSM 9957 (WP_027893118) | (2Fe–2S)-binding protein from Parvibaculum lavamentivorans DS-1Ta | WP_011995073 | 28.25 | 77 |
 Meiothermus ruber DSM 1279 (AGK03951) | Rieske (2Fe–2S) domain-containing protein from Parvibaculum lavamentivorans DS-1a | WP_011995073 | 40.68 | 76 |
 Meiothermus rufus DSM 22234 (WP_027882714) | Rieske (2Fe–2S) domain-containing protein from Parvibaculum lavamentivorans DS-1a | WP_011995073 | 26.84 | 84 |
 Meiothermus silvanus DSM 9946 (ADH64647) | Rieske (2Fe–2S) domain-containing protein from Parvibaculum lavamentivorans DS-1a | WP_011995073 | 29.83 | 80 |
 Meiothermus timidus DSM 17022 (WP_018466224) | Rieske (2Fe–2S) domain-containing protein from Parvibaculum lavamentivorans DS-1a | WP_011995073 | 27.81 | 78 |