Table 2 Purification steps of YD1
Purification steps | Vol (mL) | Specific activity (AU/mL) | Total activity (AU) | Fold | Recovery (%) |
|---|---|---|---|---|---|
Cell free supernatant | 1000 | 800 | 8 × 105 | 1 | 100 |
Ammonium sulphate | 52 | 6400 | 3.328 × 105 | 8 | 41.6 |
DEAE-Sepharose fast flow | 7 | 25,600 | 1.792 × 105 | 32 | 22.4 |
Sephadex G-25 | 3 | 32,000 | 0.96 × 105 | 40 | 12 |