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Table 4 Ranking of candidate reference genes were calculated by BestKeeper according to different expression conditions

From: Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions

Ranking ordera All conditions Different media Different light conditions
Rice medium PD medium Czapek–Dox medium All-dark All-light 12-h light/dark
1 SbVAC55 SbSAS1 TFC UBI SbVAC55 SbVAC55 UBI
CVb ± SDc 3.3 ± 0.86 1.30 ± 0.32 2.39 ± 0.70 3.64 ± 0.89 2.11 ± 0.54 3.37 ± 0.88 3.44 ± 0.83
2 SbSAS1 SbLAlv9 SbtS TFC SbSAS1 SbtS SbTRX
CV ± SD 3.60 ± 0.89 1.87 ± 0.49 2.97 ± 0.76 3.54 ± 1.07 2.40 ± 0.59 3.97 ± 1.02 3.39 ± 0.86
3 UBI SbVAC55 SbTRX SbTRX VAC UBI SbSAS1
CV ± SD 3.88 ± 0.94 1.89 ± 0.49 3.19 ± 0.82 4.02 ± 1.09 2.86 ± 0.71 4.21 ± 1.02 4.12 ± 1.01
4 SbTRX TFC SbSAS1 SbJsn1 SbtS SbSAS1 TFC
CV ± SD 3.83 ± 0.97 1.90 ± 0.53 3.43 ± 0.85 4.42 ± 1.16 2.83 ± 0.73 4.18 ± 1.04 3.72 ± 1.07
5 SbtS SbJsn1 SbVAC55 SbSAS1 SbLAlv9 TFC SbVAC55
CV ± SD 3.99 ± 1.02 2.21 ± 0.54 3.58 ± 0.93 4.86 ± 1.21 2.74 ± 0.75 4.20 ± 1.23 4.32 ± 1.12
6 TFC VAC UBI VAC SbTRX SbTRX SbLAlv9
CV ± SD 3.66 ± 1.06 2.29 ± 0.56 4.05 ± 0.99 4.75 ± 1.21 3.09 ± 0.78 5.02 ± 1.29 4.35 ± 1.17
7 SbJsn1 SbTRX SbLAlv9 SbVAC55 SbCHP SbCHP SbJsn1
CV ± SD 4.53 ± 1.15 2.48 ± 0.61 3.66 ± 1.01 4.65 ± 1.22 2.94 ± 0.81 4.75 ± 1.30 4.67 ± 1.17
8 SbLAlv9 SbtS SbJsn1 SbLAlv9 SbJsn1 SbJsn1 VAC
CV ± SD 4.28 ± 1.17 2.54 ± 0.63 3.93 ± 1.01 4.50 ± 1.25 3.59 ± 0.91 5.38 ± 1.39 4.89 ± 1.22
9 VAC SbCHP SbCHP SbCHP UBI VAC SbtS
CV ± SD 4.71 ± 1.19 2.74 ± 0.72 3.93 ± 1.08 4.88 ± 1.37 3.89 ± 0.94 5.81 ± 1.49 4.95 ± 1.26
10 SbCHP UBI VAC SbtS TFC SbLAlv9 SbCHP
CV ± SD 4.54 ± 1.24 3.26 ± 0.77 5.82 ± 1.49 5.26 ± 1.37 3.25 ± 0.95 5.69 ± 1.57 5.79 ± 1.56
  1. aReference genes were listed from the most stable to the least stable based on the values of CV and SD
  2. bCoefficient of variance expressed as a percentage on the CP level
  3. cStandard deviation of the CP