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Table 4 Ranking of candidate reference genes were calculated by BestKeeper according to different expression conditions

From: Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions

Ranking ordera

All conditions

Different media

Different light conditions

Rice medium

PD medium

Czapek–Dox medium

All-dark

All-light

12-h light/dark

1

SbVAC55

SbSAS1

TFC

UBI

SbVAC55

SbVAC55

UBI

CVb ± SDc

3.3 ± 0.86

1.30 ± 0.32

2.39 ± 0.70

3.64 ± 0.89

2.11 ± 0.54

3.37 ± 0.88

3.44 ± 0.83

2

SbSAS1

SbLAlv9

SbtS

TFC

SbSAS1

SbtS

SbTRX

CV ± SD

3.60 ± 0.89

1.87 ± 0.49

2.97 ± 0.76

3.54 ± 1.07

2.40 ± 0.59

3.97 ± 1.02

3.39 ± 0.86

3

UBI

SbVAC55

SbTRX

SbTRX

VAC

UBI

SbSAS1

CV ± SD

3.88 ± 0.94

1.89 ± 0.49

3.19 ± 0.82

4.02 ± 1.09

2.86 ± 0.71

4.21 ± 1.02

4.12 ± 1.01

4

SbTRX

TFC

SbSAS1

SbJsn1

SbtS

SbSAS1

TFC

CV ± SD

3.83 ± 0.97

1.90 ± 0.53

3.43 ± 0.85

4.42 ± 1.16

2.83 ± 0.73

4.18 ± 1.04

3.72 ± 1.07

5

SbtS

SbJsn1

SbVAC55

SbSAS1

SbLAlv9

TFC

SbVAC55

CV ± SD

3.99 ± 1.02

2.21 ± 0.54

3.58 ± 0.93

4.86 ± 1.21

2.74 ± 0.75

4.20 ± 1.23

4.32 ± 1.12

6

TFC

VAC

UBI

VAC

SbTRX

SbTRX

SbLAlv9

CV ± SD

3.66 ± 1.06

2.29 ± 0.56

4.05 ± 0.99

4.75 ± 1.21

3.09 ± 0.78

5.02 ± 1.29

4.35 ± 1.17

7

SbJsn1

SbTRX

SbLAlv9

SbVAC55

SbCHP

SbCHP

SbJsn1

CV ± SD

4.53 ± 1.15

2.48 ± 0.61

3.66 ± 1.01

4.65 ± 1.22

2.94 ± 0.81

4.75 ± 1.30

4.67 ± 1.17

8

SbLAlv9

SbtS

SbJsn1

SbLAlv9

SbJsn1

SbJsn1

VAC

CV ± SD

4.28 ± 1.17

2.54 ± 0.63

3.93 ± 1.01

4.50 ± 1.25

3.59 ± 0.91

5.38 ± 1.39

4.89 ± 1.22

9

VAC

SbCHP

SbCHP

SbCHP

UBI

VAC

SbtS

CV ± SD

4.71 ± 1.19

2.74 ± 0.72

3.93 ± 1.08

4.88 ± 1.37

3.89 ± 0.94

5.81 ± 1.49

4.95 ± 1.26

10

SbCHP

UBI

VAC

SbtS

TFC

SbLAlv9

SbCHP

CV ± SD

4.54 ± 1.24

3.26 ± 0.77

5.82 ± 1.49

5.26 ± 1.37

3.25 ± 0.95

5.69 ± 1.57

5.79 ± 1.56

  1. aReference genes were listed from the most stable to the least stable based on the values of CV and SD
  2. bCoefficient of variance expressed as a percentage on the CP level
  3. cStandard deviation of the CP