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Table 3 Ranking of candidate reference genes calculated by NormFinder according to different expression conditions

From: Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions

Ranking ordera All conditions Different media Different light conditions
Rice medium PD medium Czapek–Dox medium All-dark All-light 12-h light/dark
1 SbLAlv9 SbLAlv9 SbJsn1 SbJsn1 SbVAC55 SbJsn1 SbLAlv9
NFb 0.220 0.055 0.149 0.087 0.204 0.160 0.032
2 SbJsn1 SbSAS1 SbSAS1 VAC SbLAlv9 SbSAS1 SbSAS1
NF 0.246 0.068 0.158 0.131 0.207 0.208 0.106
3 SbSAS1 SbJsn1 SbtS SbSAS1 VAC SbtS SbJsn1
NF 0.252 0.259 0.172 0.212 0.255 0.249 0.169
4 SbtS SbVAC55 SbLAlv9 SbCHP SbTRX SbCHP UBI
NF 0.375 0.299 0.173 0.213 0.365 0.263 0.408
5 SbCHP TFC SbVAC55 SbLAlv9 SbCHP SbLAlv9 SbtS
NF 0.376 0.356 0.215 0.235 0.367 0.311 0.410
6 SbVAC55 SbCHP SbTRX UBI SbJsn1 VAC VAC
NF 0.391 0.371 0.357 0.291 0.368 0.434 0.416
7 VAC VAC UBI SbPKI SbSAS1 UBI SbVAC55
NF 0.401 0.388 0.372 0.327 0.375 0.463 0.436
8 SbTRX SbtS SbCHP SbFYVE SbtS SbVAC55 SbCHP
NF 0.434 0.430 0.453 0.340 0.415 0.472 0.439
9 UBI SbTRX SbFYVE SbTRX SbPKI SbTRX TFC
NF 0.530 0.508 0.472 0.412 0.452 0.475 0.480
10 TFC SbFYVE VAC SbVAC55 TFC SbFYVE SbTRX
NF 0.584 0.608 0.543 0.428 0.511 0.500 0.496
11 SbFYVE UBI TFC TFC UBI SbPKI SbFYVE
NF 0.621 0.759 0.637 0.444 0.699 0.726 0.577
12 SbPKI SbPKI SbPKI SbtS SbFYVE TFC SbPKI
NF 0.688 0.821 0.665 0.473 0.747 0.773 0.889
  1. aRanking of 12 candidate reference genes under different conditions from the most stable genes to the least stable genes by NF value
  2. bThe NF values were calculated by NormFinder, and the minimal NF value is considered to be the most stable