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Table 3 Ranking of candidate reference genes calculated by NormFinder according to different expression conditions

From: Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions

Ranking ordera

All conditions

Different media

Different light conditions

Rice medium

PD medium

Czapek–Dox medium

All-dark

All-light

12-h light/dark

1

SbLAlv9

SbLAlv9

SbJsn1

SbJsn1

SbVAC55

SbJsn1

SbLAlv9

NFb

0.220

0.055

0.149

0.087

0.204

0.160

0.032

2

SbJsn1

SbSAS1

SbSAS1

VAC

SbLAlv9

SbSAS1

SbSAS1

NF

0.246

0.068

0.158

0.131

0.207

0.208

0.106

3

SbSAS1

SbJsn1

SbtS

SbSAS1

VAC

SbtS

SbJsn1

NF

0.252

0.259

0.172

0.212

0.255

0.249

0.169

4

SbtS

SbVAC55

SbLAlv9

SbCHP

SbTRX

SbCHP

UBI

NF

0.375

0.299

0.173

0.213

0.365

0.263

0.408

5

SbCHP

TFC

SbVAC55

SbLAlv9

SbCHP

SbLAlv9

SbtS

NF

0.376

0.356

0.215

0.235

0.367

0.311

0.410

6

SbVAC55

SbCHP

SbTRX

UBI

SbJsn1

VAC

VAC

NF

0.391

0.371

0.357

0.291

0.368

0.434

0.416

7

VAC

VAC

UBI

SbPKI

SbSAS1

UBI

SbVAC55

NF

0.401

0.388

0.372

0.327

0.375

0.463

0.436

8

SbTRX

SbtS

SbCHP

SbFYVE

SbtS

SbVAC55

SbCHP

NF

0.434

0.430

0.453

0.340

0.415

0.472

0.439

9

UBI

SbTRX

SbFYVE

SbTRX

SbPKI

SbTRX

TFC

NF

0.530

0.508

0.472

0.412

0.452

0.475

0.480

10

TFC

SbFYVE

VAC

SbVAC55

TFC

SbFYVE

SbTRX

NF

0.584

0.608

0.543

0.428

0.511

0.500

0.496

11

SbFYVE

UBI

TFC

TFC

UBI

SbPKI

SbFYVE

NF

0.621

0.759

0.637

0.444

0.699

0.726

0.577

12

SbPKI

SbPKI

SbPKI

SbtS

SbFYVE

TFC

SbPKI

NF

0.688

0.821

0.665

0.473

0.747

0.773

0.889

  1. aRanking of 12 candidate reference genes under different conditions from the most stable genes to the least stable genes by NF value
  2. bThe NF values were calculated by NormFinder, and the minimal NF value is considered to be the most stable