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Table 1 Description of candidate reference genes, and the details of primers and amplicons

From: Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions

Gene Description Amplicon length (bp) Primer sequence (5′ → 3′) Efficiency (%) R2
SbSAS1 GTP-binding protein SAS1 141 F: 5′-GCGATTCAGCGAAGACTCCT-3′ 98 0.9998
R: 5′-ATGGTCCTGAAACGCTCCTG-3′
SbTRX 4A/4B type thioredoxin-like protein 184 F: 5′-TCAAGGCCATGTACGAGCTG-3′ 100 1
R: 5′-ACTAGACCCCTGCCCTTCTT-3′
SbtS t-SNARE 136  F: 5′-CACACAGTCCAAGTTGCAGC-3′ 97 0.9999
R: 5′-ATCGGTAGGTGATTGCGCAT-3′
SbJsn1 RNA binding protein-like protein Jsn1 175  F: 5′-TGCCCAGAAGATCATCGACG-3′ 101 0.9999
R: 5′-ACGGCCAAGCATAACCTCAA-3′
SbCHP Conserved hypothetical protein 143  F: 5′-TACGTCATTGGTGTCCGAGC-3′ 102 0.9990
R: 5′-TTGCCTCGACATGGTCTTCC-3′
SbLAlv9 LAlv9 family protein 159  F: 5′-TCCCCTCCAACAGCTCGATA-3′ 97 0.9998
R: 5′-TGACGAAGCGATGCAGAAGT-3′
SbPKI Pkinase-domain-containing protein 166  F: 5′-TGCCGCCATACTTCCAACTT-3′ 97 0.9999
R: 5′-TTATTTCCCGGAGAGCGGTG-3′
SbFYVE FYVE-domain-containing protein 167  F: 5′-GTGCAGGAGGATGGTTTGGA-3′ 97 0.9998
R: 5′-ACGCCCACACATACGACAAT-3′
SbVAC55 Vacuolar protein sorting 55 151 F: 5′-GGCTGTCTTTCGTTCTTGCG-3′ 98 0.9998
R: 5′-AAGTCATCCCGGTTAGCTGC-3′
UBI Ubiquitin-activating enzyme 131 F: 5′-ATCGCTGGTCTGAGAGGTCT-3′ 96 0.9997
R: 5′-GGGTGGAGGAAGAATTGCGA-3′
VAC Vacuolar ATPase subunit 1 157 F: 5′-CCGTCATTGTTGCCGAGAAC-3′ 99 0.9968
R: 5′-CACACCAGCAGTCTCTTCGT-3′
TFC Transcription factor TFIIIC 170 F: 5′-CAAGGCCGAACTTAGCGATC-3′ 101 0.9958
R: 5′-CCTCAGCATCACCGTCATTG-3′