Table 1 Primer pairs used for amplification of the monacolin K gene cluster in Monascus
Genes | Primer pairs | Length (bp) | Tm value |
|---|---|---|---|
mokA-F | 5′- GACCTCGGTCATCTTGGC -3′ | 18 | 59.1 |
mokA-R | 5′- TTGTTCCAAGCGGTCTTC -3′ | 18 | 57.3 |
mokB-F | 5′- AAACATCGTCACCAGTCT -3′ | 18 | 50.7 |
mokB-R | 5′- CTAAGTCGGGCATCTACC -3′ | 18 | 53.1 |
mokC-F | 5′- CAAGCTGCGAAATACACCAAGCCTC -3′ | 25 | 63.6 |
mokC-R | 5′- AGCCGTGTGCCATTCCTTGTTGTCC -3′ | 25 | 65.3 |
mokD-F | 5′- TTCATCTGCTGCTGGTAT -3′ | 18 | 59.8 |
mokD-R | 5′- AACTTCTCACCGTCAATG -3′ | 18 | 58.7 |
mokE-F | 5′- ATCGCAGGTCACGCACATCCAAGTC -3′ | 25 | 72.3 |
mokE-R | 5′- GTAAAGGCAGCCCGAGCAGCTTCAT -3′ | 25 | 71.1 |
mokF-F | 5′- GAGATCATAGTGGCCGACTGAA -3′ | 22 | 59.8 |
mokF-R | 5′- ACCGTCTCATCCAACCTCACGA -3′ | 22 | 56.1 |
mokG-F | 5′- CCAGGTAACCAACGGATTA -3′ | 19 | 56 |
mokG-R | 5′- GATCAGAGCAGTCACCAG -3′ | 18 | 52 |
mokH-F | 5′- CAGGAAATCTGGACTTACCCCATTG -3′ | 25 | 65.8 |
mokH-R | 5′- TGTTGGATTGTTGTTGGAGATATAC -3′ | 25 | 59.2 |
mokI-F | 5′- ATGTTGAATGGCAATGATGG -3′ | 20 | 60.9 |
mokI-R | 5′- CAGCGTGGGTGATGTATC -3′ | 18 | 61.7 |
GAPDH-F | 5′- CCGTATTGTCTTCCGTAAC -3′ | 19 | 55.4 |
GAPDH-R | 5′- GTGGGTGCTGTCATACTTG -3′ | 19 | 57.6 |