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Table 2 Overview of the comparative analysis of putative carbohydrate-active enzymes belong to members of various GH families targeting plant structural polysaccharides identified in HF cross rumen with other herbivore metagenomes

From: Metagenomic insights into the rumen microbial fibrolytic enzymes in Indian crossbred cattle fed finger millet straw

GH familya Major activity HF cross Jersey cowb Cowc Reindeerd Termitee Macropodf
Cellulases
 GH5 Cellulases 6.95 10.53 7.88 5.56 14.62 3.72
 GH6 Endoglucanase 0.10 0.44 0.00 0.00 0.00 0.00
 GH7 Endoglucanase 0.00 0.00 0.01 0.00 0.00 0.00
 GH9 Endoglucanase 3.16 3.51 4.32 2.11 2.35 0.00
 GH44 Endoglucanase 0.09 0.00 0.54 0.10 1.57 0.00
 GH45 Endoglucanase 0.19 0.00 0.62 0.00 1.04 0.00
 GH48 Cellobiohydrolases 0.19 0.00 0.02 0.10 0.00 0.00
 Subtotal   10.67 14.47 13.39 7.87 19.58 3.72
Endo-hemicellulases
 GH8 Endoxylanase 1.14 0.00 1.79 0.68 1.31 0.37
 GH10 Endo-1,4-β-xylanases 2.03 15.35 5.57 3.68 12.01 4.09
 GH11 Xylanases 0.29 0.00 0.90 0.16 3.66 0.00
 GH12 Xyloglucanases 0.53 0.44 0.00 0.00 0.00 0.00
 GH26 β-Mannase and xylanases 1.16 0.44 2.00 2.97 3.92 1.86
 GH28 Galacturonases 2.57 0.00 2.56 2.33 1.57 0.74
 GH53 Endo-1,4-β-galactanases 10.19 7.89 2.62 2.42 3.13 3.35
 Subtotal   17.92 24.12 15.45 12.23 25.59 10.41
Xylanoglucanases
 GH16 Xyloglucanases 2.53 0.00 2.62 2.25 0.26 1.49
 GH74 Xyloglucanases 0.45 0.00 2.09 0.85 1.83 0.37
 Subtotal   2.98 0.00 4.72 3.10 2.09 1.86
Debranching enzymes 
 GH51 α-l-Arabinofuranosidases 0.48 0.44 6.79 9.46 4.70 4.46
 GH54 α-l-Arabinofuranosidases 3.47 0.00 0.41 0.45 0.00 0.00
 GH62 α-l-Arabinofuranosidases 0.00 0.00 0.01 0.00 0.00 0.00
 GH67 α-Glucuronidases 0.98 0.00 0.65 1.43 2.61 1.86
 GH78 α-l-Rhamnosidases 3.38 5.70 6.85 6.07 0.00 9.29
 Subtotal   8.31 6.14 14.70 17.40 7.31 15.61
Oligosaccharide degrading enzymes
 GH1 β-Glucosidases 2.17 4.39 1.37 2.36 5.74 22.68
 GH2 β-Galactosidases 15.99 7.02 7.80 13.88 6.01 8.92
 GH3 β-Glucosidases 17.12 21.05 15.45 16.36 18.02 26.77
 GH29 α-l-Fucosidases 2.45 1.32 5.10 5.19 0.00 0.74
 GH35 β-Galactosidases 1.12 0.88 0.86 0.76 0.78 1.12
 GH38 α-Mannosidases 0.79 0.44 1.48 2.25 2.87 1.12
 GH39 β-Xylosidases 7.28 7.89 1.71 1.47 0.78 0.37
 GH42 β-Galactosidases 0.45 0.00 2.03 1.84 6.27 2.97
 GH43 Arabino/xylosidases 12.74 12.28 15.93 15.25 4.18 3.72
 GH52 β-Xylosidases 0.00 0.00 0.01 0.04 0.78 0.00
 Subtotal   60.11 55.26 51.75 59.40 45.43 68.40
Total   100.00 100.00 100.00 100.00 100.00 100.00
Metagenome size   1.8 Gb 0.28 Gb 268 Gb 0.30 Gb 0.062 Gb 0.054 Gb
  1. Table lists the percentages of different glycoside hydrolase families targeting plant cell wall polysaccharides and their major activities. GHs are divided into different groups based on their activity and hydrolysis of plant polysaccharides. Enzymes belonging to oligosaccharide degrading cluster were observed as the predominant class representing a major proportion of GHs
  2. aBased on CAZy data base (http://www.cazy.org)
  3. bWang et al. (2013) fed on Timothy grass hay
  4. cHess et al. (2011) fed on switch grass
  5. dPope et al. (2012) natural grazing on winter pastures, Norway
  6. eWarnecke et al. (2007) study on wood feeding termite
  7. fPope et al. (2010) fed on Timothy cannary grass and commercial pellet mix