Metagenomic insights into the rumen microbial fibrolytic enzymes in Indian crossbred cattle fed finger millet straw

Jose, V. Lyju; Appoothy, Thulasi; More, Ravi P.; Arun, A. Sha

doi:10.1186/s13568-016-0310-0

AMB Express

Table 2 Overview of the comparative analysis of putative carbohydrate-active enzymes belong to members of various GH families targeting plant structural polysaccharides identified in HF cross rumen with other herbivore metagenomes

From: Metagenomic insights into the rumen microbial fibrolytic enzymes in Indian crossbred cattle fed finger millet straw

GH family^a	Major activity	HF cross	Jersey cow^b	Cow^c	Reindeer^d	Termite^e	Macropod^f
Cellulases
GH5	Cellulases	6.95	10.53	7.88	5.56	14.62	3.72
GH6	Endoglucanase	0.10	0.44	0.00	0.00	0.00	0.00
GH7	Endoglucanase	0.00	0.00	0.01	0.00	0.00	0.00
GH9	Endoglucanase	3.16	3.51	4.32	2.11	2.35	0.00
GH44	Endoglucanase	0.09	0.00	0.54	0.10	1.57	0.00
GH45	Endoglucanase	0.19	0.00	0.62	0.00	1.04	0.00
GH48	Cellobiohydrolases	0.19	0.00	0.02	0.10	0.00	0.00
Subtotal		10.67	14.47	13.39	7.87	19.58	3.72
Endo-hemicellulases
GH8	Endoxylanase	1.14	0.00	1.79	0.68	1.31	0.37
GH10	Endo-1,4-β-xylanases	2.03	15.35	5.57	3.68	12.01	4.09
GH11	Xylanases	0.29	0.00	0.90	0.16	3.66	0.00
GH12	Xyloglucanases	0.53	0.44	0.00	0.00	0.00	0.00
GH26	β-Mannase and xylanases	1.16	0.44	2.00	2.97	3.92	1.86
GH28	Galacturonases	2.57	0.00	2.56	2.33	1.57	0.74
GH53	Endo-1,4-β-galactanases	10.19	7.89	2.62	2.42	3.13	3.35
Subtotal		17.92	24.12	15.45	12.23	25.59	10.41
Xylanoglucanases
GH16	Xyloglucanases	2.53	0.00	2.62	2.25	0.26	1.49
GH74	Xyloglucanases	0.45	0.00	2.09	0.85	1.83	0.37
Subtotal		2.98	0.00	4.72	3.10	2.09	1.86
Debranching enzymes
GH51	α-l-Arabinofuranosidases	0.48	0.44	6.79	9.46	4.70	4.46
GH54	α-l-Arabinofuranosidases	3.47	0.00	0.41	0.45	0.00	0.00
GH62	α-l-Arabinofuranosidases	0.00	0.00	0.01	0.00	0.00	0.00
GH67	α-Glucuronidases	0.98	0.00	0.65	1.43	2.61	1.86
GH78	α-l-Rhamnosidases	3.38	5.70	6.85	6.07	0.00	9.29
Subtotal		8.31	6.14	14.70	17.40	7.31	15.61
Oligosaccharide degrading enzymes
GH1	β-Glucosidases	2.17	4.39	1.37	2.36	5.74	22.68
GH2	β-Galactosidases	15.99	7.02	7.80	13.88	6.01	8.92
GH3	β-Glucosidases	17.12	21.05	15.45	16.36	18.02	26.77
GH29	α-l-Fucosidases	2.45	1.32	5.10	5.19	0.00	0.74
GH35	β-Galactosidases	1.12	0.88	0.86	0.76	0.78	1.12
GH38	α-Mannosidases	0.79	0.44	1.48	2.25	2.87	1.12
GH39	β-Xylosidases	7.28	7.89	1.71	1.47	0.78	0.37
GH42	β-Galactosidases	0.45	0.00	2.03	1.84	6.27	2.97
GH43	Arabino/xylosidases	12.74	12.28	15.93	15.25	4.18	3.72
GH52	β-Xylosidases	0.00	0.00	0.01	0.04	0.78	0.00
Subtotal		60.11	55.26	51.75	59.40	45.43	68.40
Total		100.00	100.00	100.00	100.00	100.00	100.00
Metagenome size		1.8 Gb	0.28 Gb	268 Gb	0.30 Gb	0.062 Gb	0.054 Gb

Table lists the percentages of different glycoside hydrolase families targeting plant cell wall polysaccharides and their major activities. GHs are divided into different groups based on their activity and hydrolysis of plant polysaccharides. Enzymes belonging to oligosaccharide degrading cluster were observed as the predominant class representing a major proportion of GHs
^aBased on CAZy data base (http://www.cazy.org)
^bWang et al. (2013) ^{fed on Timothy grass hay}
^cHess et al. (2011) ^{fed on switch grass}
^dPope et al. (2012) ^{natural grazing on winter pastures, Norway}
^eWarnecke et al. (2007) ^{study on wood feeding termite}
^fPope et al. (2010) ^{fed on Timothy cannary grass and commercial pellet} ^mix

Back to article page