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Table 2 K _m and V _max values of GDH towards different substrates

From: Purification and characterization of a novel glutamate dehydrogenase from Geotrichum candidum with higher alcohol and amino acid activity

Substrate	K _m (mol/L)	V _max [U (mg protein)⁻¹]	Substrate	K _m (μmol/L)	V _max [U (mg protein)⁻¹]
Methanol	–	–	Ethanol	–	–
1-propanol	–	–	NADP⁺	0.07	0.083
n-butanol	–	–	NADPH	0.13	0.182
Isobutanol	–	–	α-ketoglutarate	4.01	0.14
hexanol	20.37	16.13	Glutamate	41.74	0.87
Isoamyl alcohol	19.37	5.59	NH₄ ⁺	5.35	0.003

The K _m and V _max values for each substrate were determined by measuring the initial reaction rates at various non-saturating concentrations of the substrate in the presence of a fixed volume of enzyme
–Indicates not detected

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