Table 3 (R)-3-HB production in shake flask using different media
From: Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans
Overexpressed genes | (R)-3-HB [g L−1] | |||
|---|---|---|---|---|
Thiolase | Reductase | Shaking flask (after 48 h) | Shaking flask (maximal level) | |
YMM | YPD | |||
atoB | phaB | 0.29 ± 0.03 | 0.88 [140 h] | 2.74 [214 h] |
thl | 0.48 ± 0.01 | 2.15 [152 h] | 4.87 [214 h] | |
phaA | 0.56 ± 0.05 | 1.91 [140 h] | 3.74 [214 h] | |
AKAT1 | 0.43 ± 0.01 | 0.34 [120 h] | 2.76 [192 h] | |
AKAT2 | 0.38 ± 0.03 | 0.40 [144 h] | 1.96 [216 h] | |
AKAT4 | 0.00 | 0.00 | 0.00 | |
AKAT4 N10 | 0.46 ± 0.05 | 0.81 [144 h] | 3.22 [216 h] | |
AKAT4 N17 | 0.51 ± 0.05 | 0.53 [120 h] | 2.38 [192 h] | |
thl | AFOX2 | 0.00 | 0.00 | 0.00 |
ATPK2 | 0.00 | 0.00 | 0.00 | |
Negative control | 0.00 | 0.00 | 0.00 | |