Skip to main content

Table 3 (R)-3-HB production in shake flask using different media

From: Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans

Overexpressed genes (R)-3-HB [g L−1]
Thiolase Reductase Shaking flask (after 48 h) Shaking flask (maximal level)
YMM YPD
atoB phaB 0.29 ± 0.03 0.88 [140 h] 2.74 [214 h]
thl   0.48 ± 0.01 2.15 [152 h] 4.87 [214 h]
phaA   0.56 ± 0.05 1.91 [140 h] 3.74 [214 h]
AKAT1   0.43 ± 0.01 0.34 [120 h] 2.76 [192 h]
AKAT2   0.38 ± 0.03 0.40 [144 h] 1.96 [216 h]
AKAT4   0.00 0.00 0.00
AKAT4 N10   0.46 ± 0.05 0.81 [144 h] 3.22 [216 h]
AKAT4 N17   0.51 ± 0.05 0.53 [120 h] 2.38 [192 h]
thl AFOX2 0.00 0.00 0.00
  ATPK2 0.00 0.00 0.00
Negative control 0.00 0.00 0.00
  1. The final row is A. adeninivorans G1216/YIC104 negative control strain transformed an empty plasmid