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Table 2 Overexpressed genes for (R)-3-HB production

From: Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans

Gene

EMBL accession number

Organism

β-Ketothiolase

 phaA

LT608131

C. necator H16

 atoB

LT608129

E. coli K12

 thl

LT608130

C. acetobutylicum ATCC 824

 AKAT1

LT608124

A. adeninivorans LS3

 AKAT2

LT608125

 

 AKAT4

LT608126

 

 AKAT4 N10

LT608127

 

 AKAT4 N17

LT608128

 

Acetoacetyl-CoA reductase

  

 phaB

LT608132

C. necator H16

 AFOX2

LT632550

A. adeninivorans LS3

 ATPK2

LT632551

 
  1. N10/N17 subscript represent protein with sequence started with depicted amino acid from N-terminal compare to wild type. All bacterial genes are codon optimized sequences. The constructed strains used in this article are the result of overexpression of one of the thiolase genes and one of the reductase genes