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Table 2 Overexpressed genes for (R)-3-HB production

From: Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans

Gene EMBL accession number Organism
β-Ketothiolase
 phaA LT608131 C. necator H16
 atoB LT608129 E. coli K12
 thl LT608130 C. acetobutylicum ATCC 824
 AKAT1 LT608124 A. adeninivorans LS3
 AKAT2 LT608125  
 AKAT4 LT608126  
 AKAT4 N10 LT608127  
 AKAT4 N17 LT608128  
Acetoacetyl-CoA reductase   
 phaB LT608132 C. necator H16
 AFOX2 LT632550 A. adeninivorans LS3
 ATPK2 LT632551  
  1. N10/N17 subscript represent protein with sequence started with depicted amino acid from N-terminal compare to wild type. All bacterial genes are codon optimized sequences. The constructed strains used in this article are the result of overexpression of one of the thiolase genes and one of the reductase genes