From: Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans
Designation | Oligonucleotide sequence | Source |
---|---|---|
ORFs primers | Eurofins-Genomics | |
Akat1_start_MunI | TAGACAATTGATGGACAGACTCAACAACGTAGC | |
Akat1_stop_BamHI | GCAAGGATCCTTACTCCCGGATAAACAGAGC | |
Akat2_start_BamHI | TAGAGGATCCATGGAAAGAGCATCTAATCTTGCC | |
Akat2_stop_NotI | GCAAGCGGCCGCCTACTCCCTAATGAAGAGCG | |
Akat4_start_EcoRI | TAGAGAATTCATGTCTGTTTATATTCTTAGTGCTAAG | |
Akat4N10_start_EcoRI | TAGAGAATTCATGACACCCATTGGTTCGTTTTTGGG | |
Akat4 N17_start_EcoRI | TAGAGAATTCATGGGATCTCTGTCTTCTCAGAC | |
Akat4_stop_BamHI | GCAAGGATCCTCAAACTCTCTCAATTACAAGAGC | |
Atpk2_start_BamHI | GCAAGGATCCATGCTACGACGGGGAATTC | |
Atpk2_stop_NotI | TAGCGGCCGCTCACAACTTTGCCCCAGC | |
Afox2_start_EcoRI | TAGAGAATTCATGTCGGTGCCAACGGC | |
Afox2_stop_NotI | TAGCGGCCGCTTAAAGCTTGGCTCCTCCG | |
Two-step cloning | ||
TEF1_SpeI | TATAACTAGTTAGTAGCGCTAATCTATAATCAG | |
PHO5_BsiWI | CGGACGTACGAGCTTGCATGCCTGCAGA | |
TEF1_SpeI_BsiWI | TATACTAGTACTTCGTACGCTCGACTTCAATCTATAATCAGTC | |
PHO5_SacII_MluI | GGATCCGCGGCCGAACGCGTAGCTTGCATGCCTGCAGATTTTAATC |