Fig. 2

Enzyme activities of β-ketothiolases and acetoacetyl-CoA reductase; a 24 h cultivation on YMM-glc-NO₃ (2% glucose) using all different strains, measured after 24 h; names under horizontal axis represent adequate genes overexpressed in A. adeninivorans cells; negative control—yeast transformed with empty expression plasmid. b—Enzymatic activities depends on cultivation time; G1216/YIC104-AtoB-PhaB (half-producing strain—triangle marker) and G1216/YIC104-Thl-PhaB (the best producing strain—square marker). c Enzymatic activities of different acetoacetyl-CoA reductases, usage of NADH as a cofactor instead of NADPH