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Fig. 1 | AMB Express

Fig. 1

From: Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans

Fig. 1

Physical map of DNA transformed to auxotrophic A. adeninivorans G1216 strain for (R)-3-HB production. Fragments were obtained after linearization with AscI (fragment included d25S rDNA sequences) or SbfI (without d25S rDNA). THIOLASE is referred to atoB/thl/phaA/AKAT1/AKAT2/AKAT4/AKAT4 N10 /AKAT4 N17 genes encoding different versions of β-ketothiolases and REDUCTASE gene to phaB/ATPK2/AFOX2 encoding acetoacetyl-CoA reductases. Both genes were fused with TEF1 promoter and PHO5 terminator to obtain two expression modules

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