Fig. 8From: Antimicrobial activity and stability of the d-amino acid substituted derivatives of antimicrobial peptide polybia-MPIDetection of peptides-induced PI uptake by LSCM. Mid-logarithmic-phase C. glabrata cells were incubated with 0 µM or 5× MIC peptides for 2 h. Then, PI (50 µg/ml) was added and was further incubated for 10 min in the dark. PI uptake was detected by LSCM. The control was done without peptide. Control (a–c); polybia-MPI (d–f); D-MPI (g–i). Bars, 50 µmBack to article page