Table 2 Plasmids and strains used and constructed in this study
From: Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
Plasmids | Description | Origin |
|---|---|---|
pSP-GM1 | P TEF1 -P PGK1 bidirectional promoter (2 µm URA3) | Chen et al. (2012) |
pAB1 | pSP-GM1 P TEF1 -xfpk(AN) | This study |
pAB2 | pSP-GM1 P TEF1 -xfpk(BA) | This study |
pAB3 | pSP-GM1 P TEF1 -xfpk(BB) | This study |
pAB4 | pSP-GM1 P TEF1 -xfpk(BL) | This study |
pAB5 | pSP-GM1 P TEF1 -xfpk(CA) | This study |
pAB6 | pSP-GM1 P TEF1 -xfpk(LM) | This study |
pAB7 | pSP-GM1 P TEF1 -xfpk(LPP) | This study |
pAB8 | pSP-GM1 P TEF1 -xfpk(LP1) | This study |
pAB9 | pSP-GM1 P TEF1 -xfpk(LP2) | This study |
Strains | Genotype | Origin |
|---|---|---|
CEN.PK 113-5D | MATa MAL2-8 c SUC2 ura3-52 | P. Kötter, University of Frankfurt, Germany |
5Dgpp1∆ | MATa MAL2-8 c SUC2 ura3-52 gpp1∆ | This study |
5Dgpp2∆ | MATa MAL2-8 c SUC2 ura3-52 gpp2∆ | This study |
5Dgpp1∆gpp2∆ | MATa MAL2-8 c SUC2 ura3-52 gpp1∆ gpp2∆ | This study |
AB1 | CEN.PK 113-5D pAB1 | This study |
AB2 | CEN.PK 113-5D pAB2 | This study |
AB3 | CEN.PK 113-5D pAB3 | This study |
AB4 | CEN.PK 113-5D pAB4 | This study |
AB5 | CEN.PK 113-5D pAB5 | This study |
AB6 | CEN.PK 113-5D pAB6 | This study |
AB7 | CEN.PK 113-5D pAB7 | This study |
AB8 | CEN.PK 113-5D pAB8 | This study |
AB9 | CEN.PK 113-5D pAB9 | This study |
AB10 | CEN.PK 113-5D pSP-GM1 | This study |