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Table 2 Plasmids and strains used and constructed in this study

From: Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae

Plasmids Description Origin
pSP-GM1 P TEF1 -P PGK1 bidirectional promoter (2 µm URA3) Chen et al. (2012)
pAB1 pSP-GM1 P TEF1 -xfpk(AN) This study
pAB2 pSP-GM1 P TEF1 -xfpk(BA) This study
pAB3 pSP-GM1 P TEF1 -xfpk(BB) This study
pAB4 pSP-GM1 P TEF1 -xfpk(BL) This study
pAB5 pSP-GM1 P TEF1 -xfpk(CA) This study
pAB6 pSP-GM1 P TEF1 -xfpk(LM) This study
pAB7 pSP-GM1 P TEF1 -xfpk(LPP) This study
pAB8 pSP-GM1 P TEF1 -xfpk(LP1) This study
pAB9 pSP-GM1 P TEF1 -xfpk(LP2) This study
Strains Genotype Origin
CEN.PK 113-5D MATa MAL2-8 c SUC2 ura3-52 P. Kötter, University of Frankfurt, Germany
5Dgpp1∆ MATa MAL2-8 c SUC2 ura3-52 gpp1 This study
5Dgpp2∆ MATa MAL2-8 c SUC2 ura3-52 gpp2 This study
5Dgpp1∆gpp2∆ MATa MAL2-8 c SUC2 ura3-52 gpp1gpp2 This study
AB1 CEN.PK 113-5D pAB1 This study
AB2 CEN.PK 113-5D pAB2 This study
AB3 CEN.PK 113-5D pAB3 This study
AB4 CEN.PK 113-5D pAB4 This study
AB5 CEN.PK 113-5D pAB5 This study
AB6 CEN.PK 113-5D pAB6 This study
AB7 CEN.PK 113-5D pAB7 This study
AB8 CEN.PK 113-5D pAB8 This study
AB9 CEN.PK 113-5D pAB9 This study
AB10 CEN.PK 113-5D pSP-GM1 This study