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Table 3 CT data of reference genes calculated using Bestkeeper

From: Validation of reference genes for normalization of gene expression by qRT-PCR in a resveratrol-producing entophytic fungus (Alternaria sp. MG1)

Genes ACTB EF1 EF2 RPS5 RPS24 TUBA UBC UFD 18S
Number of sample 6 6 6 6 6 6 6 6 6
GM (CT) 20.35 25.66 21.56 20.72 20.24 21.13 22.7 23.38 16.9
AM (CT) 20.35 25.66 21.57 20.73 20.27 21.13 22.72 23.4 16.98
Min (CT) 19.57 25.19 19.95 19.86 18.19 20.52 21 22.42 14.42
Max (CT) 21.09 26.1 22.5 22.05 21.67 21.85 23.52 25.55 20.06
SD (±CT) 0.41 0.25 0.61 0.49 0.69 0.25 0.6 0.78 1.05
CV (% CT) 2.04 0.99 2.8 2.38 3.42 1.2 2.66 3.35 6.2
Min (x-fold) −1.71 −1.39 −3.04 −1.82 −4.15 −1.53 −3.25 −1.95 −5.59
Max (x-fold) 1.67 1.36 1.92 2.51 2.69 1.65 1.76 4.5 8.93
SD (x-fold) 1.33 1.19 1.52 1.41 1.62 1.19 1.52 1.72 2.08