Table 1 Relation of primers for the candidate genes to internal control
Internal gene | Gene name | Primer sequence (5′–3′) Forward/reverse | Amplicon length (bp) | Amplification efficiency (%) | Regression coefficient (R 2) | Accession number at GenBank |
|---|---|---|---|---|---|---|
ACTB | β-Actin | CAAGACGGAAGGCTGGAA/ CACTGCCGAGCGAGAAAT | 195 | 100.4 | 0.997 | GEMY01018051 |
EF1 | Elongation factor 1 | CACTGGTTTTGCCTTTTCCT/ TGTGGGCACCGTCAAAGT | 186 | 127.3 | 0.995 | GEMY01015044 |
EF2 | Elongation factor 2 | ATAACAGCCTGGAAGATGC/ CTTTCACCATCCGTCAGTT | 207 | 98.3 | 0.996 | GEMY01001243 |
RPS5 | Ribosomal protein S5 | ACACCCATACAAAGAACG/ CCGAGTGCCTTGCTGA | 131 | 104.1 | 0.985 | GEMY01011888 |
RPS24 | Ribosomal protein S24 | CCGTCTTGTCGTTCCC/ CGATTGGCGGTTTCTC | 133 | 104.8 | 0997 | GEMY01015522 |
TUBA | α-Tubulin | CAAGCGAGTCAGAAGC/ GGTATGTTGGTGAGGGTAT | 101 | 106.9 | 0.984 | GEMY01012167 |
UBC | Ubiquitin-conjugating enzyme | GGCTCAAGAAACAGGAA/ AGATTTACCACCCGAAC | 123 | 100.4 | 0.984 | GEMY01016137 |
UFD | Ubiquitin fusion degradation protein | TCCTCCTTGCCCTTGA/ CGAATCCGCCTCCTAC | 108 | 123.6 | 0.996 | GEMY01001986 |
18S | 18S ribosomal RNA | TCTTGTTTCCTTGGTGGGT/ GCATTTCGCTGCGTTCT | 144 | 106.2 | 0.980 | JN102357.1 |