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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacterium in the intestine and improves postnatal growth of chicken

Fig. 3

Relative expression of EGFP in transfected 293FT cells and antibacterial activity of supernatant of 293FT cells. a PCR amplification of lysozyme and EGFP in transfected 293FT cells. Lane 1, 2, 3, and 4 shows expression of lysozyme and EGFP in Yp group, Yh group, Yc group and control group, respectively. b qPCR detection of EGFP expression in transfected 293FT cells. c Standard curve of lysozyme activity. d Antibacterial activity against micrococcus lysodeikticus of supernatant of 293FT cell. Control group: 293FT cells without transfection; Yp group: 293FT cells transfected with pIRES2-EGFP; Yh group: 293FT cells transfected with pHmLZ-IRES2-EGFP; Yc group: 293FT cells transfected with pChLZ-IRES2-EGFP. Statistical significance is presented as follows: *P < 0.05; **P < 0.01

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