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Table 2 Substrate influence on enzyme accumulation in bioreactors

From: Myceliophthora thermophila M77 utilizes hydrolytic and oxidative mechanisms to deconstruct biomass

Run Bioreactor conditions Cellulase Xylanase β-glucosidase Protein
Substrate T(°C) pH Pulse FPase/mL p (h) IU/mL p (h) IU/mL p (h) mg/mL p(h)
#1 SCBSE 45 5 96 0.10 ± 0.01 72 3.88 ± 0.12 72 0.37 ± 0.04 120 0.06 ± 0.02 120
#2 SCBSE 45 5 48/96 0.13 ± 0.03 120 1.93 ± 0.13 96 0.30 ± 0.06 96 0.08 ± 0.02 96
#3 SCBSE 45 6 48/96 0.10 ± 0.02 96 1.80 ± 0.03 48 0.48 ± 0.03 120 0.10 ± 0.04 96
#4 SCBSE 38–29 6 96 0.18 ± 0.03 120 2.00 ± 0.01 120 0.44 ± 0.02 96 0.17 ± 0.01 120
#5 SCBSE + SUC 45 6 None 0.21 ± 0.02 24 2.70 ± 0.07 48 0.72 ± 0.01 120 0.16 ± 0.05 24
#6 WB 45 6 None 0.06 ± 0.01 24 2.88 ± 0.01 24 1.64 ± 0.10 96 0.13 ± 0.01 24
  1. SCBSE steam-exploded sugar cane bagasse; WB wheat bran; SUC sucrose; T temperature; (p), peak activity