Myceliophthora thermophila M77 utilizes hydrolytic and oxidative mechanisms to deconstruct biomass

AMB Express

Table 2 Substrate influence on enzyme accumulation in bioreactors

Run	Bioreactor conditions				Cellulase		Xylanase		β-glucosidase		Protein
Run	Substrate	T(°C)	pH	Pulse	FPase/mL	p (h)	IU/mL	p (h)	IU/mL	p (h)	mg/mL	p(h)
#1	SCBSE	45	5	96	0.10 ± 0.01	72	3.88 ± 0.12	72	0.37 ± 0.04	120	0.06 ± 0.02	120
#2	SCBSE	45	5	48/96	0.13 ± 0.03	120	1.93 ± 0.13	96	0.30 ± 0.06	96	0.08 ± 0.02	96
#3	SCBSE	45	6	48/96	0.10 ± 0.02	96	1.80 ± 0.03	48	0.48 ± 0.03	120	0.10 ± 0.04	96
#4	SCBSE	38–29	6	96	0.18 ± 0.03	120	2.00 ± 0.01	120	0.44 ± 0.02	96	0.17 ± 0.01	120
#5	SCBSE + SUC	45	6	None	0.21 ± 0.02	24	2.70 ± 0.07	48	0.72 ± 0.01	120	0.16 ± 0.05	24
#6	WB	45	6	None	0.06 ± 0.01	24	2.88 ± 0.01	24	1.64 ± 0.10	96	0.13 ± 0.01	24

SCBSE steam-exploded sugar cane bagasse; WB wheat bran; SUC sucrose; T temperature; (p), peak activity