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Table 1 Myceliophthora thermophila enzyme accumulation in shaking flask bioreactors

From: Myceliophthora thermophila M77 utilizes hydrolytic and oxidative mechanisms to deconstruct biomass

  Cellulase Xylanase β-glucosidase Protein
FPU/mL p (h) IU/mL p (h) IU/mL p (h) mg/mL p (h)
 SCBSE 0.23 ± 0.03 48 2.90 ± 0.06 48 0.43 ± 0.10 72 0.12 ± 0.01 120
 SCBDL 0.10 ± 0.01 48 2.60 ± 0.20 72 0.33 ± 0.06 96 0.07 ± 0.01 96
 WB 0.12 ± 0.02 48 2.00 ± 0.04 24 1.00 ± 0.04 120 0.15 ± 0.02 120
 EC 0.10 ± 0.01 48 2.60 ± 0.14 72 0.33 ± 0.08 96 0.04 ± 0.01 48
 SCBSE + WB 0.10 ± 0.02 72 2.80 ± 0.05 72 0.55 ± 0.05 72 0.13 ± 0.02 96
 SCBSE + SM 0.13 ± 0.02 72 2.50 ± 0.10 48 0.53 ± 0.07 120 0.24 ± 0.03 96
 SCBSE + SM 0.13 ± 0.03 72 2.80 ± 0.08 72 0.42 ± 0.05 120 0.10 ± 0.01 120
 3XSCBSE + 1XFOS   3.50 ± 0.05 48   0.11 ± 0.01 24
 1XSCBSE + 1XLAC   3.50 ± 0.10 72   0.08 ± 0.01 24
 3XSCBSE + 1XLAC 0.19 ± 0.02 72 3.10 ± 0.08 72 0.57 ± 0.05 120 0.12 ± 0.01 96
 3XSCBSE + 1XGLY 0.18 ± 0.02 96 2.80 ± 0.05 72 0.46 ± 0.09 120 0.10 ± 0.02 48
 3XSCBSE + 1XSUC 0.18 ± 0.04 96 3.30 ± 0.12 72 0.43 ± 0.08 120 0.10 ± 0.01 120
  1. SCBSE steam-exploded sugar cane bagasse; SCBDL delignified steam-exploded sugar cane bagasse; EC celuflok 200™; WB wheat bran; SM soybean mill; GLY glycerol; LAC lactose; SUC sucrose; FOS commercial phosphofructooligosacharide. Bioreactor time (h) of peak activity (p)