Myceliophthora thermophila M77 utilizes hydrolytic and oxidative mechanisms to deconstruct biomass

AMB Express

Table 1 Myceliophthora thermophila enzyme accumulation in shaking flask bioreactors

	Cellulase		Xylanase		β-glucosidase		Protein
	FPU/mL	p (h)	IU/mL	p (h)	IU/mL	p (h)	mg/mL	p (h)
SCBSE	0.23 ± 0.03	48	2.90 ± 0.06	48	0.43 ± 0.10	72	0.12 ± 0.01	120
SCBDL	0.10 ± 0.01	48	2.60 ± 0.20	72	0.33 ± 0.06	96	0.07 ± 0.01	96
WB	0.12 ± 0.02	48	2.00 ± 0.04	24	1.00 ± 0.04	120	0.15 ± 0.02	120
EC	0.10 ± 0.01	48	2.60 ± 0.14	72	0.33 ± 0.08	96	0.04 ± 0.01	48
SCBSE + WB	0.10 ± 0.02	72	2.80 ± 0.05	72	0.55 ± 0.05	72	0.13 ± 0.02	96
SCBSE + SM	0.13 ± 0.02	72	2.50 ± 0.10	48	0.53 ± 0.07	120	0.24 ± 0.03	96
SCBSE + SM	0.13 ± 0.03	72	2.80 ± 0.08	72	0.42 ± 0.05	120	0.10 ± 0.01	120
3XSCBSE + 1XFOS	–		3.50 ± 0.05	48	–		0.11 ± 0.01	24
1XSCBSE + 1XLAC	–		3.50 ± 0.10	72	–		0.08 ± 0.01	24
3XSCBSE + 1XLAC	0.19 ± 0.02	72	3.10 ± 0.08	72	0.57 ± 0.05	120	0.12 ± 0.01	96
3XSCBSE + 1XGLY	0.18 ± 0.02	96	2.80 ± 0.05	72	0.46 ± 0.09	120	0.10 ± 0.02	48
3XSCBSE + 1XSUC	0.18 ± 0.04	96	3.30 ± 0.12	72	0.43 ± 0.08	120	0.10 ± 0.01	120

SCBSE steam-exploded sugar cane bagasse; SCBDL delignified steam-exploded sugar cane bagasse; EC celuflok 200™; WB wheat bran; SM soybean mill; GLY glycerol; LAC lactose; SUC sucrose; FOS commercial phosphofructooligosacharide. Bioreactor time (h) of peak activity (p)