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Table 1 Masses, protein quantities, and Hg complexation (precipitation) of EPS extracted from the supernatants and pellets of yeast strains cultivated in media supplemented with or without HgCl2

From: Mercury removal during growth of mercury tolerant and self-aggregating Yarrowia spp.

Strain Hg conc in medium (μg ml−1) Cell bound EPS Extracellular EPS
Massa (mg dw) Hg chelated onto EPS (mg gdw−1) Protein in EPS (mg gdw−1) Massa (mgdw) Hg chelated onto EPS (mg gdw−1) Precipitated Hg in supernatant (%) Protein in EPS (mg gdw−1)
Idd1 0 0.6 0.209 0.028 3.0 0.035 NA 0.028
16.0 0.5 2.45 0.707 2.4 8.50 53.1 0.848
32.0 0.7 4.50 0.752 2.9 13.1 40.8 1.90
Idd2 0 1.2 0.316 0.028 5.0 0.073 NA 0.028
16.0 0.9 2.59 0.799 2.3 9.41 58.8 0.740
32.0 1.1 4.46 0.897 1.4 13.8 48.1 1.39
  1. Values represent mean of triplicate experiments
  2. NA means not applicable because the medium was not supplemented with HgCl2, but Hg analysis of the culture revealed Hg presence as explained in “Hg micro-precipitation due to EPS complexation with Hg” section. Growth medium (negative control) without inoculation with yeast strains lacks detectable Hg
  3. aMass of EPS extracted from 20 ml yeast culture (OD600 nm = 1.2, approx.)