Table 4 Combined assay performance for GM ELISA, Aspergillus RT-PCR, and Aspergillus NASBA-ELISA when testing cases of proven/probable IA

Sensitivity

97.22 (85.47–99.93)

55.56 (38.10–72.06)

83.33 (67.19–93.63)

55.56 (38.10–72.06)

77.78 (60.85–89.88)

52.78 (35.49–69.59)

Specificity

68.00 (53.30–80.48)

94.00 (83.45–98.75)

62.00 (47.17–75.35)

100 (92.89–100)

66.00 (51.23–78.79)

98.00 (89.35–99.95)

Positive likelihood ratio

3.04 (2.02–4.57)

9.26 (2.98–28.82)

2.19 (1.50–3.22)

Infinity

2.29 (1.50–3.49)

26.39 (3.70–188.24)

Negative likelihood ratio

0.04 (0.01–0.28)

0.47 (0.33–0.69)

0.27 (0.13–0.58)

0.44 (0.31–0.64)

0.34 (0.18–0.64)

0.48 (0.34–0.68)

PPV [% (95 % CI)]

68.63 (54.11–80.89)

86.96 (66.41–97.22)

61.22 (46.24–74.80)

100 (83.16–100)

62.22 (46.54–76.23)

95.00 (75.13–99.87)

NPV [% (95 % CI)]

97.14 (85.08–99.93)

74.60 (62.06–84.73)

83.78 (67.99–93.81)

75.76 (63.64–85.46)

80.49 (65.13–91.18)

74.24 (61.99–84.22)

Youden index

0.652

0.496

0.453

0.556

0.438

0.508