Skip to main content

Table 4 Combined assay performance for GM ELISA, Aspergillus RT-PCR, and Aspergillus NASBA-ELISA when testing cases of proven/probable IA

From: Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis

parameter NASBA-ELISA ora RT-PCR NASBA-ELISA andb RT-PCR NASBA-ELISA or GM-ELISA NASBA-ELISA and GM-ELISA RT-PCR or GM-ELISA RT-PCR and GM-ELISA
Sensitivity 97.22 (85.4799.93) 55.56 (38.10–72.06) 83.33 (67.19–93.63) 55.56 (38.10–72.06) 77.78 (60.85–89.88) 52.78 (35.49–69.59)
Specificity 68.00 (53.30–80.48) 94.00 (83.45–98.75) 62.00 (47.17–75.35) 100 (92.89100) 66.00 (51.23–78.79) 98.00 (89.35–99.95)
Positive likelihood ratio 3.04 (2.02–4.57) 9.26 (2.98–28.82) 2.19 (1.50–3.22) Infinity 2.29 (1.50–3.49) 26.39 (3.70188.24)
Negative likelihood ratio 0.04 (0.01–0.28) 0.47 (0.33–0.69) 0.27 (0.13–0.58) 0.44 (0.31–0.64) 0.34 (0.18–0.64) 0.48 (0.340.68)
PPV [% (95 % CI)] 68.63 (54.11–80.89) 86.96 (66.41–97.22) 61.22 (46.24–74.80) 100 (83.16100) 62.22 (46.54–76.23) 95.00 (75.13–99.87)
NPV [% (95 % CI)] 97.14 (85.0899.93) 74.60 (62.06–84.73) 83.78 (67.99–93.81) 75.76 (63.64–85.46) 80.49 (65.13–91.18) 74.24 (61.99–84.22)
Youden index 0.652 0.496 0.453 0.556 0.438 0.508
  1. Combined analysis for both proven IA (n = 9) and probable IA (n = 27)
  2. Best performance values are highlighted in italics
  3. a “or” indicates that at least one of the assays is required to be positive
  4. b “and” indicates that both assays are required to be positive before a patient is considered positive