Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis

AMB Express

Table 3 Performance parameters for GM ELISA, Aspergillus RT-PCR, and Aspergillus NASBA-ELISA when testing cases of proven/probable IA

Parameter	NASBA-ELISA	RT-PCR	GM-ELISA
Sensitivity [% (95 % CI)]	80.56 (63.98–91.81)	72.22 (54.81–85.80)	58.33 (40.76–74.49)*
Specificity [% (95 % CI)]	80.00 (66.28–89.97)	84.00 (70.89–92.83)	82.00 (68.56–91.42)
Positive likelihood ratio	4.03 (2.26–7.17)	4.51 (2.32–8.79)	3.24 (1.69–6.23)
Negative likelihood ratio	0.24 (0.12–0.48)	0.33 (0.19–0.57)	0.51 (0.34–0.76)
PPV [% (95 % CI)]	74.36 (57.87–86.96)	76.47 (58.83–89.25)	70.00 (50.60–85.27)
NPV [% (95 % CI)]	85.11 (71.69–93.80)	80.77 (67.47–90.37)	73.21 (59.70–84.17)
Youden index	0.61	0.56	0.40
κ statistic	0.60	0.57	0.41