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Table 3 Performance parameters for GM ELISA, Aspergillus RT-PCR, and Aspergillus NASBA-ELISA when testing cases of proven/probable IA

From: Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis

Parameter NASBA-ELISA RT-PCR GM-ELISA
Sensitivity [% (95 % CI)] 80.56 (63.9891.81) 72.22 (54.81–85.80) 58.33 (40.76–74.49)*
Specificity [% (95 % CI)] 80.00 (66.28–89.97) 84.00 (70.8992.83) 82.00 (68.56–91.42)
Positive likelihood ratio 4.03 (2.26–7.17) 4.51 (2.328.79) 3.24 (1.69–6.23)
Negative likelihood ratio 0.24 (0.12–0.48) 0.33 (0.19–0.57) 0.51 (0.340.76)
PPV [% (95 % CI)] 74.36 (57.87–86.96) 76.47 (58.8389.25) 70.00 (50.60–85.27)
NPV [% (95 % CI)] 85.11 (71.6993.80) 80.77 (67.47–90.37) 73.21 (59.70–84.17)
Youden index 0.61 0.56 0.40
κ statistic 0.60 0.57 0.41
  1. Combined analysis for both proven IA (n = 9) and probable IA (n = 27)
  2. Best performance values are highlighted in italics
  3. * P < 0.05 versus NASBA-ELISA