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Table 1 Nucleotide sequences of oligonucleotide primers and probe used in this study

From: Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis

Primers/probe Sequence (5′-3′) Length (bp)
Primers 2.1 5′-GCCGCGGTAATTCCAGCTCCAATA-3′ 24
Primers 1.2 + T7a 5′-AATTCTAATACGACTCACTATAGGGGAGCAAAGGCCTGCTTTGAACA-3′ 47
Hybridization probe Bioth-GGTCCGCCTCACCGCGAGTACTG-3′ 20
NASBA product   243
  1. aUnderlining indicates the T7 promoter region