Table 1 Nucleotide sequences of oligonucleotide primers and probe used in this study
Primers/probe | Sequence (5′-3′) | Length (bp) |
|---|---|---|
Primers 2.1 | 5′-GCCGCGGTAATTCCAGCTCCAATA-3′ | 24 |
Primers 1.2 + T7a | 5′-AATTCTAATACGACTCACTATAGGGGAGCAAAGGCCTGCTTTGAACA-3′ | 47 |
Hybridization probe | Bioth-GGTCCGCCTCACCGCGAGTACTG-3′ | 20 |
NASBA product | 243 |