Fig. 2From: Expression, purification and immobilization of tannase from Staphylococcus lugdunensis MTCC 3614Release of insert from pET 28a vector with restriction enzymes. Recombinant vector and empty vector were incubated with or without restriction enzymes used for cloning for 2 h at 37 °C. The products were separated on 1 % agarose gel. Lane 1: 1 kb marker; Lane 2: pET28a vector without enzymes; Lane 3: pET28a vector with restriction enzymes; Lane 4: Recombinant pET28a without restriction digestion; Lane 5: Recombinant pET28a vector with restriction digestionBack to article page