Disruption of Pseudomonas putida by high pressure homogenization: a comparison of the predictive capacity of three process models for the efficient release of arginine deiminase

AMB Express

Table 4 Analysis of variance for the response surface quadratic model (Eq. 8) of the extent of cell disruption

Source	SS	DF	MS	F value	P value (Prob > F)
Model	3292.93	9	365.88	67.15	<0.0001
Cell concentration (A)	170.08	1	170.08	31.22	0.0002
Operating pressure (B)	920.30	1	920.30	168.90	<0.0001
Number of passes (C)	1653.15	1	1653.15	303.41	<0.0001
AB	0.24	1	0.24	0.044	0.8378
AC	4.04	1	4.04	0.74	0.4091
BC	78.15	1	78.15	14.34	0.0036
A ²	33.88	1	33.88	6.22	0.0318
B ²	105.55	1	105.55	19.37	0.0013
C ²	128.56	1	128.56	23.60	0.0007
Residual	54.49	10	5.45
Lack-of-fit	23.64	5	4.73	0.77	0.6112
Pure error	30.84	5	6.17
Cor. total	3347.42	19

R ² = 0.9837; adjusted R ² = 0.9691; predicted R ² = 0.8711; adequate precision = 32.201; coefficient of variation (%) = 3.12; standard deviation = 2.33; mean = 74.89
SS sum of squares; DF degrees of freedom; MS mean sum of squares