Disruption of Pseudomonas putida by high pressure homogenization: a comparison of the predictive capacity of three process models for the efficient release of arginine deiminase

AMB Express

Table 3 Analysis of variance for the response surface quadratic model (Eq. 7) of total protein release

Source	SS	DF	MS	F value	P value (Prob > F)
Model	14.95	9	1.66	29.01	<0.0001
Cell concentration (A)	10.82	1	10.82	189.04	<0.0001
Operating pressure (B)	1.07	1	1.07	18.71	0.0015
No. of passes (C)	0.81	1	0.81	14.19	0.0037
AB	0.22	1	0.22	3.81	0.0796
AC	0.80	1	0.80	13.93	0.0039
BC	0.34	1	0.34	5.88	0.0358
A ²	0.12	1	0.12	2.14	0.1740
B ²	3.321 × 10⁻³	1	3.321 × 10⁻³	0.058	0.8145
C ²	0.22	1	0.22	3.88	0.0772
Residual	0.57	10	0.057
Lack-of-fit	0.27	5	0.054	0.88	0.5535
Pure error	0.30	5	0.061
Cor. total	15.52	19

R ² = 0.9631; adjusted R ² = 0.9299; predicted R ² = 0.8457; adequate precision = 19.536; coefficient of variation (%) = 7.42; standard deviation = 0.24; mean = 3.22
SS sum of squares; DF degrees of freedom; MS mean sum of squares