Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

AMB Express

Table 4 COD balance and theoretical H₂ production of C. termitidis mono-cultured and co-cultured with C. beijerinckii on 2 g l⁻¹ cellulose

		Metabolites^a (g COD l⁻¹)	H ^b₂ (g COD l⁻¹)	Cellulose (g COD l⁻¹)	Biomass^c (g COD l⁻¹)	Total COD (g COD l⁻¹)	COD balance^d (%)	Theoretical H₂ (ml)			Experimental H₂ (ml)	Difference (%)
		Metabolites^a (g COD l⁻¹)	H ^b₂ (g COD l⁻¹)	Cellulose (g COD l⁻¹)	Biomass^c (g COD l⁻¹)	Total COD (g COD l⁻¹)	COD balance^d (%)	From acetic acid	From butyric acid	Total	Experimental H₂ (ml)	Difference (%)
Mono	Initial	0.01	0	2.55	0.01	2.57	97	247	0	247	245	1
Mono	Final	1.08	0.31	0.49	0.61	2.49	97	247	0	247	245	1
Co	Initial	0.19	0	2.55	0.02	2.76	108	136	186	322	324	0
Co	Final	1.79	0.41	0.17	0.63	3	108	136	186	322	324	0

^aMetabolites COD accounts for the sum of acetate, butyrate, lactate, formate and ethanol as g COD l⁻¹
^bCalculated based on 8 g COD g⁻¹ H₂
^cBiomass COD was calculated by multiplying dry weight (g l⁻¹) × 0.9 × 1.42 (g COD g⁻¹ biomass)
^dCOD mass balance = (Final TCOD/Initial TCOD) × 100 %