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Table 4 COD balance and theoretical H2 production of C. termitidis mono-cultured and co-cultured with C. beijerinckii on 2 g l−1 cellulose

From: Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

  Metabolitesa (g COD l−1) H b2 (g COD l−1) Cellulose (g COD l−1) Biomassc (g COD l−1) Total COD (g COD l−1) COD balanced (%) Theoretical H2 (ml) Experimental H2 (ml) Difference (%)
From acetic acid From butyric acid Total
Mono Initial 0.01 0 2.55 0.01 2.57 97 247 0 247 245 1
Final 1.08 0.31 0.49 0.61 2.49
Co Initial 0.19 0 2.55 0.02 2.76 108 136 186 322 324 0
Final 1.79 0.41 0.17 0.63 3
  1. aMetabolites COD accounts for the sum of acetate, butyrate, lactate, formate and ethanol as g COD l−1
  2. bCalculated based on 8 g COD g−1 H2
  3. cBiomass COD was calculated by multiplying dry weight (g l−1) × 0.9 × 1.42 (g COD g−1 biomass)
  4. dCOD mass balance = (Final TCOD/Initial TCOD) × 100 %
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