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Fig. 1 | AMB Express

Fig. 1

From: Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79

Fig. 1

The bioinformatics analysis of ST79 peptidase M15A protein sequence. a The Peptidase_M15_3 conserved domain analyzed using Pfam; #HMM: consensus of the Hidden Markov Models (HMMs), Capital letters indicated the most conserved positions, #MATCH: the match between the query sequence and the HMM, ‘ + ’ indicates a positive score which can be interpreted as a conservative substitution, #PP: posterior probability, which is the degree of confidence in each individual aligned residue, 0 means 0–5 %, 1 means 5–15 % and so on and 9 means 85–95 %, ‘*’ means 95–100 % posterior probability, #SEQ: query sequence, ‘-’ indicated deletions in the query sequence with respect to the HMM, b Multiple alignment of peptidase protein sequences from the MEROPS database showed identity of peptidase M15A sequence from ST79 phage with peptidase enzymes from B. glumae, B. thailandensis, B. cenocepacia, Burkholderia spp. CCGE1002, A. excentricus and P. putida using the CLUSTAL X program. Conserved amino acids of all sequences were marked with an asterisk. c The full amino acids sequence analysis of ST79 peptidase M15A using MEROPS batch Blast. The peptidase unit was labeled in green, active site in red, metal ion ligand in orange and the conserved domain in underlined letters

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