NFAP2, a novel cysteine-rich anti-yeast protein from Neosartorya fischeri NRRL 181: isolation and characterization

AMB Express

Table 1 In vitro minimal inhibitory concentration (MIC) values of NFAP2, NFAP and conventional antifungal agents for the investigated fungal isolates

Fungus	MIC (µg ml⁻¹)
Fungus	NFAP2	NFAP	AMB	ITC	FLC	TRB	CSP
Yeasts
Candida albicans ATCC 10231	0.781	>200	8	>64	>64	64	16
Candida glabrata CBS 138	0.391	>200	8	>64	>64	64	16
Candida guilliermondii CBS 566	0.391	>200	4	2	8	16	16
Candida krusei CBS 573	1.563	>200	8	2	>64	64	16
Candida lusitaniae CBS 6936	0.781	>200	8	16	>64	16	16
Candida parapsilosis CBS 604	0.391	>200	8	2	>64	16	32
Candida tropicalis CBS 94	0.391	>200	8	>64	>64	>64	8
Saccharomyces cerevisiae SZMC 0644	0.195	>200	4	2	64	>64	16
Schizosaccharomyces pombe SZMC 0142	0.391	>200	4	8	64	>64	16
Filamentous fungi
Aspergillus nidulans FGSC A4	>25	200	>64	4	>64	8	>64
Aspergillus niger SZMC 601	>25	50	8	8	>64	16	>64
Rhizomucor mieheii CBS 360.92	>25	>200	2	1	16	8	8

MIC was determined after 24 h (yeasts and R. miehei CBS 360.92) and 48 h (Aspergillus spp.) of incubation. Abbreviations: AMB amphotericin B, CSP caspofungin, FLC fluconazole, ITC itraconazole, NFAP Neosartorya fischeri antifungal protein, NFAP2 Neosartorya fischeri antifungal protein 2, TRB terbinafine