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Table 3 The enrichment of the phage in during the bio-panning

From: Bioscreening and expression of a camel anti-CTGF VHH nanobody and its renaturation by a novel dialysis–dilution method

library Input phage (pfu) Output (pfu) Gene polymorphism loci ina Calculated library sizeb
CDR1 CDR2 CDR3
VHH library NA NA 7 12 86 NA
1st panning 3 × 1010 1.36 × 105 3 8 15 6.71 × 107
2nd panning 4 × 109 3.19 × 106 NA NA NA NA
3rd panning 2 × 107 1.92 × 106 4 4 3 3072
  1. NA undetected or unmeasured
  2. aLoci where gene sequencing results indicating presence of different nucleotides
  3. bThe calculated size should be much greater than actually size due to gene coupling