Table 3 The enrichment of the phage in during the bio-panning
library | Input phage (pfu) | Output (pfu) | Gene polymorphism loci ina | Calculated library sizeb | ||
|---|---|---|---|---|---|---|
CDR1 | CDR2 | CDR3 | ||||
VHH library | NA | NA | 7 | 12 | 86 | NA |
1st panning | 3 × 1010 | 1.36 × 105 | 3 | 8 | 15 | 6.71 × 107 |
2nd panning | 4 × 109 | 3.19 × 106 | NA | NA | NA | NA |
3rd panning | 2 × 107 | 1.92 × 106 | 4 | 4 | 3 | 3072 |