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Table 2 The panning conditions for each round

From: Bioscreening and expression of a camel anti-CTGF VHH nanobody and its renaturation by a novel dialysis–dilution method

Panning rounds Each well Rinse times
Input phage (pfu/ml) Tween-20 (%) CTGF-C (μg)
1st 3 × l011 0.1 2 5
2nd 4 × 1010 0.5 2 10
3rd 2 × 108 0.5 2 15