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Table 2 The panning conditions for each round

From: Bioscreening and expression of a camel anti-CTGF VHH nanobody and its renaturation by a novel dialysis–dilution method

Panning rounds

Each well

Rinse times

Input phage (pfu/ml)

Tween-20 (%)

CTGF-C (μg)

1st

3 × l011

0.1

2

5

2nd

4 × 1010

0.5

2

10

3rd

2 × 108

0.5

2

15