From: Bioscreening and expression of a camel anti-CTGF VHH nanobody and its renaturation by a novel dialysis–dilution method
Panning rounds
Each well
Rinse times
Input phage (pfu/ml)
Tween-20 (%)
CTGF-C (μg)
1st
3 × l011
0.1
2
5
2nd
4 × 1010
0.5
10
3rd
2 × 108
15