Relative significances of pH and substrate starch level to roles of Streptococcus bovis S1 in rumen acidosis

AMB Express

Table 3 Effects of soluble starch concentrations and pH on organic acids production

Items	PH	Concentration (g/L)			Mean_pH	SEM_Total	P _pH	P _C	P _pH×C
Items	PH	1	3	9	Mean_pH	SEM_Total	P _pH	P _C	P _pH×C
Formate (mM)¹	6.5	1.25	1.14	0.58	0.99	0.843	0.077	0.135	<0.001
	5.5	0.48	0.92	1.21	0.87
	Mean_C	0.87	1.03	0.90
Acetate (mM)¹	6.5	4.15	3.83	2.05	3.34	0.210	0.701	<0.001	0.093
	5.5	3.57	4.25	2.00	3.28
	Mean_C	3.86^a	4.04^a	2.03^b
Lactate (mM)1	6.5	14.66	34.28	56.01	34.98^a	1.950	0.001	<0.001	0.641
	5.5	9.83	26.62	47.63	28.03^b
	Mean_C	12.25^c	30.45^b	51.82^a
OA_total (mM)²	6.5	20.07	39.24	58.65	39.32^a	2.114	0.001	<0.001	0.962
	5.5	13.85	31.80	50.85	32.16^b
	Mean_C	16.96^c	35.52^b	54.75^a
OA_per (mM)³	6.5	20.07	13.08	8.38	13.84^a	0.591	<0.001	<0.001	<0.001
	5.5	13.85	10.60	10.17	11.54^b
	Mean_C	16.96^a	11.84^b	9.27^c
Formate (%)⁴	6.5	6.26	2.87	1.00	3.38^a	0.175	0.003	<0.001	<0.001
	5.5	3.23	2.92	2.37	2.84^b
	Mean_C	4.74^a	2.90^b	1.68^c
Acetate (%)⁴	6.5	20.63	9.82	3.50	11.32^b	1.031	0.003	<0.001	0.102
	5.5	25.83	13.42	3.93	14.39^a
	Mean_C	23.23^a	11.62^b	3.72^c
Lactate (%)⁴	6.5	73.12	87.31	95.50	85.31^a	0.929	0.006	<0.001	0.586
	5.5	70.94	83.66	93.70	82.77^b
	Mean_C	72.03^c	85.48^b	94.60^a