Table 3 Mutants with reduced adhesion or invasion capabilities and the affected genes
Mutants | Length obtained (bp) | Gene and locus tag | Source | Putative function | Identities (%) |
|---|---|---|---|---|---|
AM 1-3 | 184 | recB, AFK64_02315 | C. sakazakii, NCTC 8155 | Exonuclease V subunit beta | 177/184 (96) |
AM 4 | 510 | bglF, AFK64_20760 | C. sakazakii, NCTC 8155 | PTS sugar transporter (beta-glucoside component) | 493/510 (97) |
AM 5 | 340 | fnr, AFK64_08415 | C. sakazakii, NCTC 8155 | Fumarate and nitrate reduction regulatory protein | 337/340 (99) |
AM 6 | 323 | flhA, AFK64_06715 | C. sakazakii, NCTC 8155 | Flagellar biosynthesis protein FlhA | 320/323 (99) |
AM 7 | 315 | fliR, ESA_02516 | C. sakazakii, ES15 | Flagellar biosynthesis protein FliR | 313/315 (99) |
AM 8 | 94 | hp-1 a, AFK64_01330 | C. sakazakii, NCTC 8155 | Hypothetical protein | 91/94 (97) |
AM 9 | 190 | hp-2 a, ESA_04202 | C. sakazakii, ATCC BAA-894 | Hypothetical protein | 189/190 (99) |
AM 10 | 772 | hp-3 a, ESA_00132 | C. sakazakii, ATCC BAA-894 | Hypothetical protein | 759/772 (98) |