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Table 6 Substrate protection studies

From: Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

Amino acid Reaction system Residual activity (%)
Carboxylic acid Buffer + enzyme 100.00
Buffer + enzyme +200 mM EDAC 69.93
Buffer + enzyme + 1.84 mM pNPX 75.50
Buffer + enzyme + 3.68 mM pNPX 76.72
Tyrosine Buffer + enzyme 100.00
Buffer + enzyme +100 mM NAI 52.99
Buffer + enzyme + 1.84 mM pNPX + 100 mM NAI 61.05
Buffer + enzyme + 3.68 mM pNPX + 100 mM NAI 61.75
Tryptophan Buffer + enzyme 100.00
Buffer + enzyme +100 µM NBS 0.79
Buffer + enzyme + 1.84 mM pNPX + 100 µM NBS 43.29
Buffer + enzyme + 3.68 mM pNPX + 100 µM NBS 67.07
  1. The PhXyl was pre-incubated with excess of pNPX in the respective buffers of modifying reagents. After 10 min of incubation at room temperature, the suitable concentration of modifying reagents was added and incubated for further 30 min. The aliquots were removed for the determination of residual enzyme activity under standard assay conditions. The values are the average of three independent experiments with 3-5 % standard deviation