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AMB Express

Table 6 Substrate protection studies

From: Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

Amino acid

Reaction system

Residual activity (%)

Carboxylic acid

Buffer + enzyme

100.00

Buffer + enzyme +200 mM EDAC

69.93

Buffer + enzyme + 1.84 mM pNPX

75.50

Buffer + enzyme + 3.68 mM pNPX

76.72

Tyrosine

Buffer + enzyme

100.00

Buffer + enzyme +100 mM NAI

52.99

Buffer + enzyme + 1.84 mM pNPX + 100 mM NAI

61.05

Buffer + enzyme + 3.68 mM pNPX + 100 mM NAI

61.75

Tryptophan

Buffer + enzyme

100.00

Buffer + enzyme +100 µM NBS

0.79

Buffer + enzyme + 1.84 mM pNPX + 100 µM NBS

43.29

Buffer + enzyme + 3.68 mM pNPX + 100 µM NBS

67.07

  1. The PhXyl was pre-incubated with excess of pNPX in the respective buffers of modifying reagents. After 10 min of incubation at room temperature, the suitable concentration of modifying reagents was added and incubated for further 30 min. The aliquots were removed for the determination of residual enzyme activity under standard assay conditions. The values are the average of three independent experiments with 3-5 % standard deviation
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