Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

AMB Express

Table 5 Effect of group specific modifying agents on PhXyl activity

Modifying agent	Concentration (mM)	Possible amino acid modification	Buffer systems	Residual activity (%)^a
EDAC	200	Asx/Glx	MES/HEPES, 75:25, 50 mM pH 6	69
DEPC	5	His	Sodium phosphate 50 mM, pH 6	100
NBS	0.1	Try	Sodium acetate 50 mM, pH 4.5	00
NEM	50	Cys	Sodium phosphate 50 mM, pH 7.5	100
Iodoacetate	5	Cys	Sodium phosphate 50 mM, pH 8	100
NAI	50	Tyr	Sodium borate 50 mM, pH 7.6	62
PMSF	5	Ser	Sodium phosphate 50 mM, pH 7.5	100
Phenylglyoxal	5	Arg	Sodium bicarbonate 50 mM, pH 8.5	100
Citraconic anhydride	5	Lys	Sodium bicarbonate 50 mM, pH 8.4	100
Trinitrobenzene sulfonic acid	5	Lys	Sodium bicarbonate (4 %) 100 mM, pH 8.4	100