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Table 5 Effect of group specific modifying agents on PhXyl activity

From: Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

Modifying agent

Concentration (mM)

Possible amino acid modification

Buffer systems

Residual activity (%)a

EDAC

200

Asx/Glx

MES/HEPES, 75:25, 50 mM pH 6

69

DEPC

5

His

Sodium phosphate 50 mM, pH 6

100

NBS

0.1

Try

Sodium acetate 50 mM, pH 4.5

00

NEM

50

Cys

Sodium phosphate 50 mM, pH 7.5

100

Iodoacetate

5

Cys

Sodium phosphate 50 mM, pH 8

100

NAI

50

Tyr

Sodium borate 50 mM, pH 7.6

62

PMSF

5

Ser

Sodium phosphate 50 mM, pH 7.5

100

Phenylglyoxal

5

Arg

Sodium bicarbonate 50 mM, pH 8.5

100

Citraconic anhydride

5

Lys

Sodium bicarbonate 50 mM, pH 8.4

100

Trinitrobenzene sulfonic acid

5

Lys

Sodium bicarbonate (4 %) 100 mM, pH 8.4

100

  1. aThe mean values show the average of three independent experiments