Skip to main content

Table 5 Effect of group specific modifying agents on PhXyl activity

From: Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

Modifying agent Concentration (mM) Possible amino acid modification Buffer systems Residual activity (%)a
EDAC 200 Asx/Glx MES/HEPES, 75:25, 50 mM pH 6 69
DEPC 5 His Sodium phosphate 50 mM, pH 6 100
NBS 0.1 Try Sodium acetate 50 mM, pH 4.5 00
NEM 50 Cys Sodium phosphate 50 mM, pH 7.5 100
Iodoacetate 5 Cys Sodium phosphate 50 mM, pH 8 100
NAI 50 Tyr Sodium borate 50 mM, pH 7.6 62
PMSF 5 Ser Sodium phosphate 50 mM, pH 7.5 100
Phenylglyoxal 5 Arg Sodium bicarbonate 50 mM, pH 8.5 100
Citraconic anhydride 5 Lys Sodium bicarbonate 50 mM, pH 8.4 100
Trinitrobenzene sulfonic acid 5 Lys Sodium bicarbonate (4 %) 100 mM, pH 8.4 100
  1. aThe mean values show the average of three independent experiments