Modifying agent | Concentration (mM) | Possible amino acid modification | Buffer systems | Residual activity (%)a |
---|---|---|---|---|
EDAC | 200 | Asx/Glx | MES/HEPES, 75:25, 50 mM pH 6 | 69 |
DEPC | 5 | His | Sodium phosphate 50 mM, pH 6 | 100 |
NBS | 0.1 | Try | Sodium acetate 50 mM, pH 4.5 | 00 |
NEM | 50 | Cys | Sodium phosphate 50 mM, pH 7.5 | 100 |
Iodoacetate | 5 | Cys | Sodium phosphate 50 mM, pH 8 | 100 |
NAI | 50 | Tyr | Sodium borate 50 mM, pH 7.6 | 62 |
PMSF | 5 | Ser | Sodium phosphate 50 mM, pH 7.5 | 100 |
Phenylglyoxal | 5 | Arg | Sodium bicarbonate 50 mM, pH 8.5 | 100 |
Citraconic anhydride | 5 | Lys | Sodium bicarbonate 50 mM, pH 8.4 | 100 |
Trinitrobenzene sulfonic acid | 5 | Lys | Sodium bicarbonate (4 %) 100 mM, pH 8.4 | 100 |